高效液相色谱法测定不同类别食品中的叶黄素  

Determination of lutein in different types of foods by high performance liquid chromatography

作　　者：张志军[1,2] 张飞 付强[1,2] 杨熙 林奕云[1,2] 钟永红 黄兴民[1] ZHANG Zhi-jun;ZHANG Fei;FU Qiang;YANG Xi;LIN Yi-yun;ZHONG Yong-hong;HUANG Xing-min(China National Analytical Center (Guangzhou),Guangdong 510070,China;Guangdong Provincial Key Laboratory of Emergency Test for Dangerous Chemicals,Guangdong 510070,China)

机构地区：[1]中国广州分析测试中心,广东广州510070 [2]广东省化学危害应急检测技术重点实验室,广东广州510070

出　　处：《广西大学学报（自然科学版）》2019年第3期869-875,共7页Journal of Guangxi University（Natural Science Edition）

基　　金：广东省自然科学基金资助项目(2015A030310466);广东省科技计划项目(2016A040403061);广东省科学院科技创新专项(2017GDASCX-0104)

摘　　要：为了研究不同类别食品中不同形态叶黄素的分析方法,针对不同样品基质和叶黄素形态,分别选用超声波辅助提取法、酶解—超声波提取法和碱皂化—液液萃取法,建立并优化了测定叶黄素的高效液相色谱法。采用C18柱(4.6mm×250mm,5μm)进行检测,以甲醇—水为流动相进行等度洗脱,流速为1.0mL/min,柱温为35℃,检测波长为446nm,叶黄素在0.125~10.0mg/L范围内呈良好线性,相关系数为0.99991。不同类别食品的加标回收率为90.0%~102.2%,方法检出限为0.10mg/kg。采用该方法对各类食品样品中叶黄素的含量进行了测定,实验结果表明该方法适用范围广、针对性强、准确度高和重现性好,适用于各类食品中叶黄素的检测。In order to study the method for the determination of different forms lutein in various foods,different pretreatment methods were selected according to sample matrix and forms of lutein,including ultrasound-assisted extraction method,enzymatic hydrolysis-ultrasonic method and saponification-extraction method.A method for the determination of lutein by high performance liquid chromatography was established and optimized. HPLC separation of lutein was conducted on a C 18 column (4.6 mm×250 mm,5 μm ) with the mobile phase of methanol and water at the flow rate of 1.0 mL/min and the column temperature was 35 ℃. The detection was carried out at a wavelength of 446 nm.The linear range of the method for lutein were 0.125~10.0 mg/L with correlation coefficients not less than 0.999 91 .The recoveries of the lutein in various foods were from 90.0 % to 102.2 %.The detection limit of the lutein was 0.10 mg/kg.This method was applied in the investigation of lutein in various foods.The method has wide applicability,pertinence,good accuracy and reproducibility and is suitable for the detection of lutein in various foods.

关 键 词：叶黄素 叶黄素微囊粉 叶黄素酯 食品 高效液相色谱法 

分 类 号：O657.7[理学—分析化学]