Trichothecium roseum菌丝体细胞壁提取物对苹果青霉病的控制效果及其诱导抗病机理  

Controlling Effect and Mechanism of Trichothecium roseum Hyphal Cell Wall Extract on Apple Blue Mold

作　　者：张彦东 胡文瑾[1] 李永才 毕阳[1] 张婷婷[1] 胡培芳 ZHANG Yandong;HU Wenjin;LI Yongcai;BI Yang;ZHANG Tingting;HU Peifang(College of Food Science and Engineering,Gansu Agricultural University,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学食品科学与工程学院

出　　处：《食品科学》2019年第13期230-236,共7页Food Science

基　　金：国家自然科学基金地区科学基金项目(31460534)

摘　　要：以‘红富士’苹果为试材,通过粉红单端孢(Trichothecium roseum)菌丝体细胞壁提取物处理后24 h损伤接种扩展青霉(Penicillium expansum),研究其对苹果果实青霉病的抑制效果,并通过生化方法进一步揭示了T. roseum菌丝体细胞壁提取物的部分诱导抗病机理。结果表明,T. roseum菌丝体细胞壁提取物处理能有效抑制损伤接种P. expansum苹果青霉病的扩展,其中以150 mg/mL T. roseum菌丝体细胞壁提取物处理效果最好,处理后第5天病害程度相比于对照组降低了29.5%。进一步研究表明,150 mg/mL T. roseum菌丝体细胞壁提取物处理显著提高了贮藏期间果实组织中多酚氧化酶、过氧化物酶、苯丙氨酸解氨酶、4-香豆酰-辅酶A连接酶、肉桂酸-4-羟化酶和肉桂醇脱氢酶的活力(P<0.05),同时提高了果实组织中总酚、类黄酮和木质素的含量。进一步推断出T. roseum菌丝体细胞壁提取物可通过促进苯丙烷代谢、提高抗性相关酶的活力和抗性物质的积累而增加果实的抗病性。Fuji apple fruit were arti ficially wounded and inoculated with Penicillium expansum (blue mold) at 24 h after being treated with a hyphal cell wall extract from Trichothecium roseum so as to evaluate the control of the extract on apple blue mold and to partially elucidate the underlying biochemical mechanism. The results showed that the treatment of the cell wall extract could effectively inhibit the development of inoculated P. expansum in apple wounds, and the best effect was observed at a concentration of 150 mg/mL, resulting in a reduction in disease severity of 29.5% after 5 d storage when compared with the control group. Further studies showed that the activity of polyphenol oxidase (PPO), peroxidase (POD), phenylalanine ammonia lyase (PAL), 4-coumaryl coenzyme A ligase (4CL), cinnamic acid-4-hydroxylase (C4H) and cinnamyl alcohol dehydrogenase (CAD) was significantly increased by the treatment of the extract at 150 mg/mL (P < 0.05). At the same time, the contents of total phenols, flavonoids and lignin in fruit tissues were also increased. These findings suggested that the hyphal cell wall extract from T. roseum might enhance disease resistance by promoting the metabolism of phenylpropane, and increasing the activity of resistance-related enzyme activities and the accumulation of antifungal compounds.

关 键 词：粉红单端孢 细胞壁提取物 苹果 青霉病 诱导抗病性 

分 类 号：TS255.36[轻工技术与工程—农产品加工及贮藏工程]