Localization Analysis of Heterophilic Antigen Epitopes of H1N1 Influenza Virus Hemagglutinin  

作　　者：Chun-Yan Guo Hai-Xiang Zhang Jun-Jun Zhang Li-Jun Sun Hui-Jin Li Dao-Yan Liang Qing Feng Yan Li Yang-Meng Feng Xin Xie Jun Hu 

机构地区：[1]Shaanxi Province Research Center of Cell Immunological Engineering and Technology,Central Laboratory of Shaanxi Provincial People's Hospital,Xi'an 710068,China [2]Xian Yang Municipal Center for Disease Control and Prevention,Xianyang 712000,China [3]Shaanxi Key Laboratory of Ischemic Cardiovascular Disease,Institute of Basic and Translational Medicine,Xi'an Medical University,Xi'an 710021,China [4]Key Laboratory of Resource Biology and Biotechnology in Western China,Ministry of Education,College of Life Sciences,Northwest University,Xi'an 710069,China

出　　处：《Virologica Sinica》2019年第3期306-314,共9页中国病毒学（英文版）

基　　金：supported by The National Key Research and Development Program of China (Grant No. 2016YFD0500700);The Natural Science Basic Research Program of Shaanxi Province (Grant No. 2016JM8065);Shaanxi Provincial People’s Hospital Incubation Fund Program (Grant No. 2015YX-4)

摘　　要：Previous studies have indicated that two monoclonal antibodies(mAbs;A1-10 and H1-84)of the hemagglutinin(HA)antigen on the H1N1 influenza virus cross-react with human brain tissue.It has been proposed that there are heterophilic epitopes between the HA protein and human brain tissue(Guo et al.in Immunobiology 220:941-946,2015).However,characterisation of the two mAbs recognising the heterophilic epitope on HA has not yet been performed.In the present study,the common antigens of influenza virus HA were confirmed using indirect enzyme-linked immunosorbent assays and analysed with DNAMAN software.The epitopes were localized to nine peptides in the influenza virus HA sequence and the distribution of the peptides in the three-dimensional structure of HA was determined using PyMOL software.Key amino acids and variable sequences of the antibodies were identified using abYsis software.The results demonstrated that there were a number of common antigens among the five influenza viruses studied that were recognised by the mAbs.One of the peptides,P2(LVLWGIHHP191-199),bound both of the mAbs and was located in the head region of HA.The key amino acids of this epitope and the variable regions in the heavy and light chain sequences of the mAbs that recognised the epitope are described.A heterophilic epitope on H1N1 influenza virus HA was also introduced.The existence of this epitope provides a novel perspective for the occurrence of nervous system diseases that could be caused by influenza virus infection,which might aid in influenza prevention and control.Previous studies have indicated that two monoclonal antibodies(mAbs; A1-10 and H1-84) of the hemagglutinin(HA)antigen on the H1 N1 influenza virus cross-react with human brain tissue. It has been proposed that there are heterophilic epitopes between the HA protein and human brain tissue(Guo et al. in Immunobiology 220:941–946, 2015). However,characterisation of the two mAbs recognising the heterophilic epitope on HA has not yet been performed. In the present study, the common antigens of influenza virus HA were confirmed using indirect enzyme-linked immunosorbent assays and analysed with DNAMAN software. The epitopes were localized to nine peptides in the influenza virus HA sequence and the distribution of the peptides in the three-dimensional structure of HA was determined using PyMOL software. Key amino acids and variable sequences of the antibodies were identified using abYsis software. The results demonstrated that there were a number of common antigens among the five influenza viruses studied that were recognised by the mAbs. One of the peptides, P2(LVLWGIHHP191–199), bound both of the mAbs and was located in the head region of HA. The key amino acids of this epitope and the variable regions in the heavy and light chain sequences of the mAbs that recognised the epitope are described. A heterophilic epitope on H1 N1 influenza virus HA was also introduced. The existence of this epitope provides a novel perspective for the occurrence of nervous system diseases that could be caused by influenza virus infection, which might aid in influenza prevention and control.

关 键 词：H1N1 influenza virus HA ANTIGEN MONOCLONAL antibody LOCALIZATION Heterophilic EPITOPE 

分 类 号：Q[生物学]