Arabidopsis AGC protein kinases IREH1 and IRE3 control root skewing  

作　　者：Xiaozhen Yue Zhiai Guo Teng Shi Lizhen Song Youfa Cheng 

机构地区：[1]Key Laboratory of Plant Molecular Physiology,Institute of Botany,Chinese Academy of Sciences,Beijing 100093,China [2]University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Journal of Genetics and Genomics》2019年第5期259-267,共9页遗传学报（英文版）

基　　金：supported by the grants from the National Natural Science Foundation of China (31270330, 91217310, 91017008 and 31171389);the National Basic Research Program of China (2014CB943400);the National Key Research and Development Program of China (2016YFD0100402);the Strategic Priority Research Program of the Chinese Academy of Sciences (XDA0801010402);the One-Hundred Talent Project of the Chinese Academy of Sciences to Y.C

摘　　要：AGC protein kinases play important roles in plant growth and development.Several AGC kinases in Arabidopsis have been functionally characterized.However,the"AGC Other"subfamily,including IRE,IREH1,IRE3 and IRE4,has not been well understood.Here,we reported that ireh1 mutants displayed a root skewing phenotype,which can be enhanced by ire3 mutation.IREH1 and IRE3 were expressed in roots,consistent with their function in controlling root skewing.The fluorescence intensities of the microtubule marker KNpro:EGFP-MBD were decreased in ireh1,ire3 and ireh1 ire3 mutants compared to wild type.The microtubule arrangements in ireh1 and ireh1 ire3 mutants were also altered.IREH1 physically interacted with IRE3 in vitro and in planta.Thus,our findings demonstrate that IREH1 and IRE3 protein kinases play important roles in controlling root skewing,and maintaining microtubule network in Arabidopsis.AGC protein kinases play important roles in plant growth and development. Several AGC kinases in Arabidopsis have been functionally characterized. However, the "AGC Other" subfamily, including IRE,IREH1, IRE3 and IRE4, has not been well understood. Here, we reported that ireh1 mutants displayed a root skewing phenotype, which can be enhanced by ire3 mutation. IREH1 and IRE3 were expressed in roots, consistent with their function in controlling root skewing. The fluorescence intensities of the microtubule marker KNpro:EGFP-MBD were decreased in ireh1, ire3 and ireh1 ire3 mutants compared to wild type. The microtubule arrangements in ireh1 and ireh1 ire3 mutants were also altered. IREH1 physically interacted with IRE3 in vitro and in planta. Thus, our findings demonstrate that IREH1 and IRE3 protein kinases play important roles in controlling root skewing, and maintaining microtubule network in Arabidopsis.

关 键 词：AGC KINASES IREH1 IRE3 ROOT SKEWING MICROTUBULE ARABIDOPSIS 

分 类 号：R[医药卫生]