富硒短小芽孢杆菌D1-019的筛选与鉴定（英文）  

Screening, Identification and Characterization of Selenium-Enriched Bacillus pumilus D1-019

作　　者：孙毓 肖益新 张冉 王成华[1] 刘小玲[1] 赵谋明[1] SUN Yu;XIAO Yixin;ZHANG Ran;WANG Chenghua;LIU Xiaoling;ZHAO Mouming(College of Light Industry and Food Engineering,Guangxi University,Nanning 530004,China)

机构地区：[1]广西大学轻工与食品工程学院

出　　处：《食品科学》2019年第14期130-137,共8页Food Science

基　　金：“十三五”国家重点研发计划重点专项(2018YFD0400105);广西创新驱动发展专项(AA17204075;桂科AA17202010-3)

摘　　要：将无机硒耐受性筛选和"红硒法"筛选相结合,经过4轮筛选,从前期分离的119株来源于鸭食的菌株中,筛选到1株富硒菌株D1-019。基于菌落形态观察、革兰氏染色、16SrDNA序列分析和系统发育树分析,D1-019菌株被鉴定为益生菌短小芽孢杆菌。采用正交试验设计方法,获得的最佳富硒条件为培养基初始pH5,30℃培养48h,培养6 h时加入质量浓度为20μg/mL的亚硒酸钠。在该富硒条件下,短小芽孢杆菌D1-019对培养基中亚硒酸钠的转化率为100%,菌体有机硒含量为(1 327±113)mg/kg,优于已报道富硒微生物。结果表明,短小芽孢杆菌D1-019有潜力开发成为一个可应用于食品领域的新富硒益生菌源。In this study, out of 119 bacterial isolates from duck foods, a novel selenium-enriched probiotic strain, designated as D1-019, was selected for its tolerance to selenite. This strain was obtained after four rounds of screening, which did not cause a red selenium phenomenon. Strain D1-019 was identified as Bacillus pumilus based on its colonial morphology, Gram staining, 16 S rDNA analysis, and phylogenic tree analysis. Using an orthogonal experiment design, we obtained the optimum culture conditions for selenium enrichment as follows: pH 5, 30 ℃ for 48 h, and addition of 20 μg/mL selenite at 6 h of cultivation. Under these conditions, B. pumilus D1-019 eliminated selenite in the medium with a conversion rate of 100% and produced(1 327 ± 113) mg/kg of organic selenium, which was superior to the currently available seleniumenriched microbes. Our results indicated that B. pumilus D1-019 can be a promising candidate for selenium-enriched probiotics in the food industry.

关 键 词：短小芽孢杆菌 有机硒 富硒 益生菌 亚硒酸钠 16S RDNA 

分 类 号：TS254[轻工技术与工程—水产品加工及贮藏工程]