常压室温等离子体诱变选育木聚糖酶高产菌及其酶学性质研究  

Mutation breeding and enzymatic property of high yield xylanase strain by atmospheric and room temperature plasma

作　　者：朱慧霞 方桢 鲁旭峰 汪水玲 马晓静 姚日生 ZHU Huixia;FANG Zhen;LU Xufeng;WANG Shuiling;MA Xiaojing;YAO Risheng(School of Food and Biological Engineering,Hefei University of Technology,Hefei 230009,China;Engineering Research Center of Bio-process,Ministry of Education,Hefei 230009,China)

机构地区：[1]合肥工业大学食品与生物工程学院,安徽合肥230009 [2]农产品生物化工教育部工程研究中心,安徽合肥230009

出　　处：《中国酿造》2019年第7期31-36,共6页China Brewing

基　　金：国家高技术研究发展计划“863计划”项目(SS2014AA021902);国家自然科学基金青年科学基金(31400049);安徽省教育厅自然科学研究项目重点项目(非产学研)(2013AJZR0044)

摘　　要：利用常压室温等离子体(ARTP)技术对黑曲霉(Aspergillus niger)FXY进行诱变处理,并对菌株的遗传稳定性及所产木聚糖酶的酶学性质进行了研究。结果表明,筛得一株高产木聚糖酶且能稳定遗传的菌株ARTP-82,其所产木聚糖酶酶活为175.33U/mL,较出发菌株提高了120.8%。酶活的提高与酶比活力增加有关、与菌株生物量增加无关;该木聚糖酶的最适温度为45.0℃、最适pH值为7.0,在该温度和pH值条件下酶的稳定性良好。The Aspergillus niger FXY was mutated by atmospheric and room temperature plasma (ARTP),and the hereditary stability of the strain and the enzymatic properties of the xylanase were studied. The results showed that a strain ARTP-82 with high yield xylanase and stable hereditary was screened,and the xylanase activity was 175.33 U/ml,which was 120.8% higher than that of the original strain. The reason for the increase in xylanase activity was related to the increase of enzyme specific activity,instead of the increase of strain biomass. The optimum temperature and pH of xylanase were 45.0 ℃ and 7.0,respectively,and the stability of the enzyme under the condition was good.

关 键 词：黑曲霉 木聚糖酶 常压室温等离子体 酶学性质 

分 类 号：Q815[生物学—生物工程]