Identification and characterization of a novel group of natural antisense transcripts from RNA1.2 gene locus of human cytomegalovirus  

作　　者：Bing-Yang Liu Yan-Ping Ma Ying Qi Yu-Jing Huang Zhong-Yang Liu Meng-Qing Lu Qiang Ruan 

机构地区：[1]Department of Endocrinology,Affiliated Shengjing Hospital,China Medical University,Shenyang,Liaoning 110004,China [2]Virus Laboratory,Affiliated Shengjing Hospital,China Medical University,Shenyang,Liaoning 110004,China

出　　处：《Chinese Medical Journal》2019年第13期1591-1598,共8页中华医学杂志（英文版）

基　　金：grants from the National Natural Science Foundation of China(Nos.81672028 and 81371788);the Outstanding Scientific Fund of Shengjing Hospital.

摘　　要：Background:Natural anti-sense transcripts(NATs),which are transcribed from the complementary DNA strand of annotated genes,exert regulatory function of gene expression.Increasing studies recognized anti-sense transcription widespread throughout human cytomegalovirus(HCMV)genome,whereas the anti-sense transcription of RNA1.2 gene locus has never been investigated.In this study,the transcription of the RNA1.2 anti-sense strand was investigated in clinically isolated HCMV strain.Methods:Strand-specific high-through RNA-sequencing(RNA-seq)was performed to find possible anti-sense transcripts(ASTs).For analyzing and visualization of RNA-seq data sets,Integrative Genomics Viewer software was applied.To confirm these possibilities,Northern blotting and rapid amplification of cDNA ends(RACE)were used.Results:Transcription of the opposite strand of RNA1.2 gene locus was detected by RNA-sequencing using RNAs extracted from human embryonic lung fibroblasts infected with HCMV clinical isolate HAN.At least three HCMV NATs,named RNA1.2 AST 1,RNA1.2 AST2,and RNA 1.2 AST3,were characterized by Northern blotting and RACE analyses.These RNA1.2 ASTs orientated from the complementary strand of RNA1.2 locus during the late phase of HCMV infection.The 5'-and 3'-termini of these transcripts were located within the opposite sequence of the predicted RNA1.2 gene.Conclusion:A cluster of novel NATs was transcribed from the opposite sequence of the HCMV RNA 1.2 gene region.

关 键 词：HUMAN CYTOMEGALOVIRUS RNA1.2 NATURAL anti-sense TRANSCRIPT 

分 类 号：R[医药卫生]