RSBP15 interacts with and stabilizes dRSPH3 during sperm axoneme assembly in Drosophila  

作　　者：Ya Wang Rui Xu Yiwei Cheng Haowei Cao Zibin Wang Tianyu Zhu Jiayin Jiang Hao Zhang Chang Wang Lin Qi Mingxi Liu Xuejiang Guo Juan Huang Jiahao Sha 

机构地区：[1]State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, 211166, China [2]Department of Histology and Embryology, Nanjing Medical University, Nanjing, 211166, China [3]Department of Medical Genetics, Nanjing Medical University, Nanjing, 211166, China [4]Jiangsu Key Laboratory of Xenotransplantation, Nanjing Medical University, Nanjing, 211166, China

出　　处：《Journal of Genetics and Genomics》2019年第6期281-290,共10页遗传学报（英文版）

基　　金：supported by the National Basic Research Program of China (2016YFA0500903, 2016YFA0503300, and 2017YFA0103803);the National Natural Science Foundation of China (31530047 and 81771641);the Fok Ying Tung Education Foundation (161037);the 333 Project of Jiangsu Province (BRA2016386);the Program for Distinguished Talents of Six Domains in Jiangsu Province (YY-019)

摘　　要：Flagellum in sperm is composed of over 200 different proteins and is essential for sperm motility. In particular, defects in the assembly of the radial spoke in the flagellum result in male infertility due to loss of sperm motility. However, mechanisms regulating radial spoke assembly remain unclear in metazoans.Here, we identified a novel Drosophila protein radial spoke binding protein 15(RSBP15) which plays an important role in regulating radial spoke assembly. Loss of RSBP15 results in complete lack of mature sperms in seminal vesicles(SVs), asynchronous individualization complex(IC) and defective "9 + 2"structure in flagella. RSBP15 is colocalized with dRSPH3 in sperm flagella, and interacts with dRSPH3 through its DD_R_PKA superfamily domain which is important for the stabilization of dRSPH3. Moreover,loss of dRSPH3, as well as dRSPH1, dRSPH4 a and dRSPH9, showed similar phenotypes to rsbp15 KO mutant. Together, our results suggest that RSBP15 acts in stabilizing the radial spoke protein complex to anchor and strengthen the radial spoke structures in sperm flagella.Flagellum in sperm is composed of over 200 different proteins and is essential for sperm motility. In particular, defects in the assembly of the radial spoke in the flagellum result in male infertility due to loss of sperm motility. However, mechanisms regulating radial spoke assembly remain unclear in metazoans.Here, we identified a novel Drosophila protein radial spoke binding protein 15(RSBP15) which plays an important role in regulating radial spoke assembly. Loss of RSBP15 results in complete lack of mature sperms in seminal vesicles(SVs), asynchronous individualization complex(IC) and defective "9 + 2"structure in flagella. RSBP15 is colocalized with dRSPH3 in sperm flagella, and interacts with dRSPH3 through its DD_R_PKA superfamily domain which is important for the stabilization of dRSPH3. Moreover,loss of dRSPH3, as well as dRSPH1, dRSPH4 a and dRSPH9, showed similar phenotypes to rsbp15 KO mutant. Together, our results suggest that RSBP15 acts in stabilizing the radial spoke protein complex to anchor and strengthen the radial spoke structures in sperm flagella.

关 键 词：RSBP15 RSPH3 FLAGELLUM Radial SPOKE DROSOPHILA 

分 类 号：R[医药卫生]