壳聚糖浸泡对黄豆芽生长和主要生物活性物质含量的影响  

Effect of Chitosan Soaking on the Growth and Contents of Major Bioactive Substances of Soybean Sprouts

作　　者：吕霞敏 杨睿 蒋誉 黄建颖[1] 董丽娟[1] Lü Xiamin;YANG Rui;JIANG Yu;HUANG Jianying;DONG Lijuan(School of Food Science and Biotechnology,Zhejiang Gongshang University,Hangzhou 310018,China)

机构地区：[1]浙江工商大学食品与生物工程学院

出　　处：《食品科学》2019年第15期112-117,共6页Food Science

基　　金：浙江省公益事业项目(2017C32008)

摘　　要：为研究不同质量浓度壳聚糖浸泡处理对黄豆芽生长和主要活性物质含量的影响,将黄豆经不同质量浓度(0.2、0.4 g/100 mL和0.8 g/100 mL)壳聚糖溶液浸泡处理后培养成豆芽,测定其下胚轴长度和鲜质量,并利用分光光度法和液相色谱法测定其生物活性物质含量。结果表明,壳聚糖处理可以增加黄豆芽的下胚轴长度和鲜质量,并对其主要生物活性物质含量产生影响。其中,以0.8 g/100 mL壳聚糖浸泡处理并培养5 d时,黄豆芽的下胚轴长度和鲜质量显著增加(P<0.05),总黄酮含量最高(0.64 mg/g),植酸含量最低(1.56 mg/g),总抗坏血酸和总酚含量也在适宜含量范围内。结论:壳聚糖浸泡处理培养黄豆芽可以考虑作为一种生产低成本功能性食品的替代技术。In order to explore the influence of chitosan soaking on the growth and the contents of major bioactive substances of soybean sprouts, soybean was soaked in chitosan solutions at concentrations of 0.2, 0.4 and 0.8 g/100 mL and then cultured until sprouts came out. Hypocotyl length and fresh mass were measured, and the contents of bioactive substances were determined by spectrophotometry and liquid chromatography. The results showed that chitosan treatment could increase the hypocotyl length and fresh mass and simultaneously affect the contents of major bioactive substances of soybean sprouts.After being soaked with 0.8 g/100 mL chitosan and cultured for 5 days, the hypocotyl length and fresh mass of soybean sprouts significantly increased(P < 0.05);the total flavonoid content reached the highest level(0.64 mg/g), and the phytic acid content was at the lowest level(1.56 mg/g). Meanwhile, the contents of total ascorbic acid and total phenols were at the appropriate level. This study provides a theoretical basis for the application of chitosan soaking to produce low-cost functional foods.

关 键 词：壳聚糖 黄豆 发芽 生物活性物质 

分 类 号：TS255.36[轻工技术与工程—农产品加工及贮藏工程]