水豆豉中高产豆豉纤溶酶的菌株筛选、鉴定及生长性能研究  

Research on Screening,Identification and Growth Performance of High-yield Douchi Fibrinolytic Enzyme Strain from Fermented Soybeans

作　　者：董科 陈宇航 沈丹芸 裴晓方 DONG Ke;CHEN Yu-hang;SHEN Dan-yun;PEI Xiao-fang(West China School of Public Health (West China Fourth Hospital),Sichuan University,Chengdu 610041,China;Key Laboratory of Food Safety Monitoring and Risk Assessment in Sichuan Province,Chengdu 610041,China)

机构地区：[1]四川大学华西公共卫生学院(华西第四医院),成都610041 [2]食品安全监测与风险评估四川省重点实验室,成都610041

出　　处：《中国调味品》2019年第9期85-89,共5页China Condiment

基　　金：四川省科技厅科技支撑项目(2016SZ0034)

摘　　要：采用平板法初筛,从市售水豆豉分离细菌中筛选得到6株具有高产蛋白酶、淀粉酶和豆豉纤溶酶的菌株;经化学法复筛,获得1株高产豆豉纤溶酶菌株B61。对B61菌株进行形态学、生理生化特性鉴定及16S rDNA同源性分析,最后鉴定为贝莱斯芽孢杆菌(Bacillus velezensis),且该菌株在生长16 h后检测到最高豆豉纤溶酶活力(826.25 IU/mL)。该研究丰富了豆豉纤溶酶产生菌的菌种资源,并为其后续相关研究提供了理论依据。Six strains of high-yield protease,amylase and douchi fibrinolytic enzyme are screened from the isolated bacteria of commercial fermented soybeans by plate method.A strain B61 with high-yield douchi fibrinolytic enzyme is screened from fermented soybeans by chemical method.The strain B61 is identified as Bacillus velezensis by morphological,physiological and biochemical characteristics identification and 16S rDNA homologous analysis.The highest douchi fibrinolytic activity(826.25 IU/mL)is detected after growing for 16 h.This study has enriched the strain resources of douchi fibrinolytic enzyme producing bacteria,and provided theoretical basis for the follow-up related research.

关 键 词：水豆豉 豆豉纤溶酶 筛选 菌种鉴定 

分 类 号：TS201.57[轻工技术与工程—食品科学]