不同氮源对脱胚玉米挤出物发酵生产柠檬酸的影响  

The influence of different nitrogen sources on citric acid production from degermed corn extrudate

作　　者：叶向库 申德超[3] YE Xiangku;SHEN Dechao(Agricultural University of Hebei,Baoding 071001,China;Yantai University,Yantai 264005,China;Shandong University of Technology,Zibo 255091,China)

机构地区：[1]河北农业大学,河北保定071001 [2]烟台大学,山东烟台264005 [3]山东理工大学,山东淄博255091

出　　处：《食品与发酵工业》2019年第16期233-236,共4页Food and Fermentation Industries

基　　金：山东理工大学创新团队(4080-108001);横向课题基金(9194-10907701);淄博汇创生物科技有限公司经费资助

摘　　要：为了选择合适的氮源,提高使用脱胚玉米进行柠檬酸发酵的产率,该文将脱胚玉米经低温加酶挤压处理得到脱胚玉米挤出物,作为发酵原料用于柠檬酸生产.使用该挤出物制备发酵液,采用黑曲霉Co827作为发酵菌种,添加无机氮源((NH4)2 SO4、NH4 NO3、NH4 Cl)或有机氮源(尿素、玉米渣)进行柠檬酸发酵.结果表明,无机氮源中添加质量浓度3 g/L的(NH4)2 SO4促进产酸的效果较好,有机氮源中添加质量浓度1 g/L糖化后玉米渣效果较好.由发酵产酸的效果可知,脱胚玉米经低温加酶挤压处理后制备柠檬酸发酵液,在同等条件下添加糖化后玉米渣有机氮源(柠檬酸产率9.82 g/100 g)优于添加(NH4)2 SO4的无机氮源(柠檬酸产率7.86 g/100 g)的发酵效果.上述研究为提高脱胚玉米生产柠檬酸的产率、降低生产成本、提高玉米综合利用率的研究提供参考.Citric acid production from degermed corn extrudate was investigated. The purpose was to raise citric acid productivity under different nitrogen sources. The extrudate fermentation broth was acquired for citric acid production by Aspergillus niger Co827 under inorganic((NH 4) 2SO 4,NH 4NO 3,NH 4Cl) or organic(urea,corn cinder) nitrogen sources. The test results showed: It was favorable for citric acid production under 3 g/L (NH 4) 2SO 4 inorganic nitrogen sources or 1 g/L corn cinder organic nitrogen sources;Citric acid production adding corn cinder (citric acid productivity 9.82 g/100 g) was more than one adding (NH 4) 2SO 4 (citric acid productivity 7.86g/100 g). To conclude,the above results provide useful data for raising citric acid productivity from degermed corn,cutting down production costs and increasing corn’s comprehensive utilization degree.

关 键 词：脱胚玉米挤出物 柠檬酸发酵 无机氮源 有机氮源 

分 类 号：TQ921.1[轻工技术与工程—发酵工程]