Simultaneous Determination of Six Constituents in Cangbaiqutong Capsule by HPLC Wavelength Switching Technique  

作　　者：Jing WU Hongyan LIU Bo YU Zhong JIA 

机构地区：[1]Chinese Medicine Hospital of Lanzhou City, Lanzhou 730050, China [2]Lanzhou Pulmonary Hospital, Lanzhou 730046, China [3]The Second People s Hospital of Lanzhou, Lanzhou 730050, China

出　　处：《Medicinal Plant》2019年第4期20-23,共4页药用植物：英文版

基　　金：Supported by Lanzhou Talent Innovation and Entrepreneurship Science and Technology Plan Project(2015-RC-22)

摘　　要：[Objectives] To establish a high performance liquid chromatography (HPLC) wavelength switching method for the simultaneous determination of content of six constituents (phellodendrine chloride, gentiopicrin, paeoniflorin, tetrandrine, berberine hydrochloride and paeonol) in Cangbaiqutong capsules, and provide a scientific basis for the comprehensive evaluation of the quality of Cangbaiqutong capsule.[Methods] The chromatographic column of Waters XSELECT CSH-C 18 (4.6 mm × 150 mm, 5 μm), the mobile phase of acetonitrile-0.1% phosphate solution, gradient elution (0-15 min,10%-18% A;15-30 min,18%-50% A;30-35 min, 50%-10% A);the flow rate of 1.0 mL/min, wavelength switching of 284 (0-7 min, phellodendrine), 274 (7-10 min, gentiopicrin), 230 (10-14 min, paeoniflorin) and 274 nm (14-35 min, tetrandrine, berberine hydrochloride, paeonol), the injection volume of 10 μL.[Results] There was a good linear relationship between the area of chromatographic peak and the injection volume of phellodendrine chloride, gentiopicrin, paeoniflorin, tetrandrine, berberine hydrochloride and paeonol in the range of 0.150-1.504, 0.768-7.680, 1.096-10.960, 0.220-2.200, 0.296-2.956, 0.0345-0.345 μg, respectively;the average recovery rates ( n =6) were 98.3%, 99.2%, 98.8%, 98.8%, 99.1% and 98.2%, respectively;the RSD value was 1.32%, 1.46%, 1.08%, 1.31%, 1.26% and 1.21%, respectively.[Conclusions] The method can be used to determine many kinds of constituents at the same time, and the operation is simple, accurate and reproducible, and can be used for the quality control of compound Cangbaiqutong capsules.[Objectives] To establish a high performance liquid chromatography（HPLC） wavelength switching method for the simultaneous determination of content of six constituents（phellodendrine chloride, gentiopicrin, paeoniflorin, tetrandrine, berberine hydrochloride and paeonol） in Cangbaiqutong capsules, and provide a scientific basis for the comprehensive evaluation of the quality of Cangbaiqutong capsule. [Methods] The chromatographic column of Waters XSELECT CSH-C18（4.6 mm × 150 mm, 5 μm）, the mobile phase of acetonitrile-0.1% phosphate solution, gradient elution（0-15 min,10%-18% A;15-30 min,18%-50% A; 30-35 min, 50%-10% A）; the flow rate of 1.0 mL/min, wavelength switching of 284（0-7 min, phellodendrine）, 274（7-10 min, gentiopicrin）, 230（10-14 min, paeoniflorin） and 274 nm（14-35 min, tetrandrine, berberine hydrochloride, paeonol）, the injection volume of 10 μL. [Results] There was a good linear relationship between the area of chromatographic peak and the injection volume of phellodendrine chloride, gentiopicrin, paeoniflorin, tetrandrine, berberine hydrochloride and paeonol in the range of 0.150-1.504, 0.768-7.680, 1.096-10.960, 0.220-2.200, 0.296-2.956, 0.0345-0.345 μg, respectively; the average recovery rates（n=6） were 98.3%, 99.2%, 98.8%, 98.8%, 99.1% and 98.2%, respectively; the RSD value was 1.32%, 1.46%, 1.08%, 1.31%, 1.26% and 1.21%, respectively. [Conclusions] The method can be used to determine many kinds of constituents at the same time, and the operation is simple, accurate and reproducible, and can be used for the quality control of compound Cangbaiqutong capsules.

关 键 词：HPLC Cangbaiqutong CAPSULE Wavelength switching Simultaneous determination Phellodendrine CHLORIDE Gentiopicrin PAEONIFLORIN TETRANDRINE Quality control 

分 类 号：R[医药卫生]