Effect of Tong Xie Yao Fang on endogenous metabolites in urine of irritable bowel syndrome model rats  

作　　者：Xue-Ying Zhao Jian-Wei Wang Yue Yin Kai Li Miao Zhang Fu-Ping Yan 

机构地区：[1]School of Basic Medical Sciences, Heilongjiang University of Chinese Medicine

出　　处：《World Journal of Gastroenterology》2019年第34期5134-5151,共18页世界胃肠病学杂志（英文版）

基　　金：Supported by the National Natural Science Foundation of China,No.81573870;the Eighth Special Subsidy Project of China Post Doctoral Science Foundation,No.2015T80376;Postdoctoral Science Research Developmental Foundation of China,No.2013M531079;National Science Foundation of Heilongjiang Province,No.H2015020;Heilongjiang University of Traditional Chinese Medicine Outstanding Innovative Talents Support Project(Outstanding Young Academic Leaders),Postdoctoral Science-Research Developmental Foundation of Heilongjiang Province,No.LBHQ12009;Youth Academic Backbone Fund of Heilongjiang Province Education Department,No.1253G053;Youth Science and Technology Project of Traditional Chinese Medicine Department of Heilongjiang Province,No.ZQG-034

摘　　要：BACKGROUND Tong Xie Yao Fang is a representative traditional Chinese prescription for the treatment of liver and spleen deficiency,abdominal pain and diarrhea.It has a unique function in the treatment of gastrointestinal dysfunction including irritable bowel syndrome(IBS),is a common functional bowel disease.Its main symptoms are recurrent abdominal pain,diarrhea,constipation or alternations between diarrhea and constipation.There are obvious differences in metabolites between TCM syndromes.By comparing the body fluid metabolism maps of model animals,metabolomics can discover disease biomarkers,analyze the differences in metabolic pathways and understand the pathological process and the metabolic pathways of substances in the body.Thus,the evaluation of animal models tends to be comprehensive and objective.This may provide further understanding between the interaction between Tong Xie Yao Fang and the IBS model.AIM To evaluate the effect of Tong Xie Yao Fang on IBS rats by using metabolomics method.METHODS Wistar rats were used to establish IBS models,and then randomly divided into four groups:A model control group and three Tong Xie Yao Fang treatment groups(high,medium and low doses).A normal,non-IBS group was established.The rats were treated for 2 wk.On days 0 and 14 of the experimental model,urine was collected for 12 h and was analyzed by ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry.Nine potential biomarkers were identified,and six major metabolic pathways were found to be related to IBS.RESULTS In the study of metabonomics,nine potential biomarkers including L-serine,4-methylgallic acid,L-threonine,succinylacetone,prolyl-hydroxyproline,valylserine,acetyl citrate,marmesin rutinoside and 5-hydroxy-L-tryptophan were identified in urine,which were assigned to amino acids,organic acids,succinyl and glycosides.Furthermore,the metabolic pathway of L-serine,L-threonine and 5-hydroxy-L-tryptophan was found in the Kyoto Encyclopedia of Genes and Genomes,which mainly involved thBACKGROUND Tong Xie Yao Fang is a representative traditional Chinese prescription for the treatment of liver and spleen deficiency, abdominal pain and diarrhea. It has a unique function in the treatment of gastrointestinal dysfunction including irritable bowel syndrome(IBS), is a common functional bowel disease. Its main symptoms are recurrent abdominal pain, diarrhea, constipation or alternations between diarrhea and constipation.There are obvious differences in metabolites between TCM syndromes. By comparing the body fluid metabolism maps of model animals, metabolomics can discover disease biomarkers, analyze the differences in metabolic pathways and understand the pathological process and the metabolic pathways of substances in the body. Thus, the evaluation of animal models tends to be comprehensive and objective. This may provide further understanding between the interaction between Tong Xie Yao Fang and the IBS model.AIM To evaluate the effect of Tong Xie Yao Fang on IBS rats by using metabolomics method.METHODS Wistar rats were used to establish IBS models, and then randomly divided into four groups: A model control group and three Tong Xie Yao Fang treatment groups(high, medium and low doses). A normal, non-IBS group was established.The rats were treated for 2 wk. On days 0 and 14 of the experimental model,urine was collected for 12 h and was analyzed by ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry. Nine potential biomarkers were identified, and six major metabolic pathways were found to be related to IBS.RESULTSIn the study of metabonomics, nine potential biomarkers including L-serine, 4-methylgallic acid, L-threonine, succinylacetone, prolyl-hydroxyproline, valylserine, acetyl citrate, marmesin rutinoside and 5-hydroxy-L-tryptophan were identified in urine, which were assigned to amino acids, organic acids, succinyl and glycosides. Furthermore, the metabolic pathway of L-serine, L-threonine and5-hydroxy-L-tryptophan was found in the Kyoto Encyclopedia of Genes and

关 键 词：Tong Xie YAO Fang IRRITABLE BOWEL syndrome Liver-spleen disharmony ENDOGENOUS METABOLITES Metabolomics 

分 类 号：R[医药卫生]