响应面法优化提取油茶籽壳棕色素与抗氧化活性研究  

Optimization of Extraction of Brown Pigment from Camellia Seed Shell by Response Surface Methodology and Its Antioxidant Activity

作　　者：张宽朝[1] 张琛[1] 阮飞[1] 何孔泉[1] 杨超 Zhang Kuanchao;Zhang Chen;Ruan Fei;He Kongquan;Yang Chao(School of Life Science, Anhui Agriculture University, Hefei 230036)

机构地区：[1]安徽农业大学生命科学学院

出　　处：《中国粮油学报》2019年第8期126-132,共7页Journal of the Chinese Cereals and Oils Association

摘　　要：为综合开发利用油茶资源,采用响应面法优化油茶籽壳棕色素提取工艺。以提取温度、液料比、乙醇浓度、提取时间为自变量,以油茶籽壳棕色素提取含量为响应值,进行响应面分析,并研究油茶籽壳棕色素体外抗氧化活性。结果表明,影响油茶籽壳棕色素提取含量高低的因素主次顺序为提取时间>乙醇浓度>温度>液料比。油茶籽壳棕色素最佳提取工艺条件为提取温度35 ℃、液料比11 ∶ 1、乙醇体积分数65%、提取时间2 h。在此条件下,油茶籽壳棕色素提取率为15.09%,与预测值基本一致。体外抗氧化活性试验表明,油茶籽壳棕色素有一定的清除O2^-·、H2O2 的能力和还原能力。In order to comprehensively develop and utilize the Camellia oleifera resources, response surface methodology was used to optimize the brown pigment extraction process of Camellia seed shell. Extraction temperature, liquid to material ratio, ethanol concentration, and extraction time as independent variables, and the brown pigment extraction content of Camellia seed shell were taken as the response value, the response surface analysis was performed and the antioxidant activity of brown pigment was studied in vitro. The results showed that the order of the factors affecting the content of the brown pigment extract from Camellia seed shell was extraction time>ethanol concentration>temperature> liquid-material ratio. The optimum extraction conditions of brown pigment from Camellia seed shell were at 35 ℃ as extraction temperature,liquid-material ratio was 11∶ 1, ethanol concentration was 65%, and extraction time was 2h. Under this condition, the extraction rate of brown pigment from Camellia seed shell was 15.09%, which was basically consistent with the predicted value. The antioxidant activity test in vitro showed that the brown pigment of Camellia seed shell has a certain ability to remove O2^-·,H2O2 and the reducing capacity. Brown pigment from Camellia seed shell can be further studied as a natural food pigment with developing potential.

关 键 词：油茶籽壳 棕色素 响应面分析 抗氧化活性 

分 类 号：TS202.3[轻工技术与工程—食品科学]