耐热木聚糖酶菌株的筛选鉴定及发酵条件分析  

Screening and Identification of Heat-Resistant Xylanase-Producing Strain and Investigation of Fermentation Conditions

作　　者：杨然[1,2] 张笑雨 刘朋肖 范光森 李秀婷[1,3] YANG Ran;ZHANG Xiaoyu;LIU Pengxiao;FAN Guangsen;LI Xiuting(Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University,Beijing 100048, China;Beijing Key Laboratory of Flavor Chemistry, Beijing 100048, China;Beijing Higher Institution Engineering Research Center of Food Additives and Ingredients, Beijing 100048, China)

机构地区：[1]北京食品营养与人类健康高精尖创新中心(北京工商大学),北京100048 [2]北京市食品风味化学重点实验室,北京100048 [3]食品添加剂与配料北京高校工程研究中心,北京100048

出　　处：《食品科学》2019年第18期101-107,共7页Food Science

基　　金：国家自然科学基金青年科学基金项目（31501416）;北京市自然科学基金青年科学基金项目（6164029）

摘　　要：从山东淄博酒曲中筛选得到1株产耐热木聚糖酶菌株FSD0302,经鉴定为疏绵状丝孢菌(Thermomyceslanuginosus)。对该菌进行单因素优化产酶条件考察,结果显示:当玉米芯木聚糖粒度20~40目、质量浓度4 g/100 mL、初始培养基pH 6.0、发酵温度50℃、转速200 r/min时,T.lanuginosus FSD0302所产木聚糖酶活力高达5 357.1 U/mL,比活力为10 493.72 U/mg,较初筛时产酶量提高了2.9倍;该菌可产2种木聚糖酶,分子质量分别约为20 kDa和60 kDa,酶学性质考察结果表明,该菌所产木聚糖酶粗酶的最适温度为75℃,最适pH值分别为5.5及7.5且在pH 3.5~9.0范围内可保留50%以上酶活力。综上所述,来源于T.lanuginosus FSD0302的耐热木聚糖酶可在低聚木糖生产中具有一定的应用前景。In this research, we isolated a thermostable xylanase-producing strain FSD0302 from Jiuqu (a traditional Chinese liquor fermentation starter) from Zibo, Shandong, and we identi fied it as Thermomyces lanuginosus. The fermentation conditions for the production of xylanase by this strain were investigated by one-factor-at-a-time method. The results showed that a higher xylanase activity of 5 357.1 U/mL was obtained under the following conditions: particle size of corncob xylan 20 40 mesh, xylan concentration 4 g/100 mL, initial medium pH 6.0, fermentation temperature 50 ℃, and rotational speed 200 r/min, which was 3.9 times as much as that before the optimization. The specific activity was calculated as 10 493.72 U/mg. Strain FSD0302 produced two xylanases with different molecular masses of about 20 and 60 kDa, respectively. The enzymatic properties showed that the optimum temperature was 75 ℃ for both xylanases, and the optimum pH was 5.5 and 7.5, respectively. Over 50% of the xylanase activity could be retained in the pH range of 3.5 9.0. To sum up, the thermostable xylanase from T. lanuginosus FSD0302 is promising for application in the production of xylooligosaccharides.

关 键 词：疏绵状丝孢菌 耐热木聚糖酶 发酵条件 酶学性质 

分 类 号：Q814[生物学—生物工程]