Study on immunological characteristics of T lymphocyte in peripheral blood from patients with sjogren's syndrome  

作　　者：Rong Bi Sheng-Ping Zeng Bo Wen Chun-Bo Wang Ji-Hong Tan 

机构地区：[1]Affiliated Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, China [2]Affiliated Hospital of Chengdu University of Traditional Chinese Medicine/Chengdu Gaoxin Huisheng Rheumatism Hospital, Chengdu, China [3]Beijing Traditional Chinese Medicine Hospital Affiliated to Capital Medical University, Chengdu, China [4]Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Chengdu, China [5]Chengdu Yingdu District Hospital of Traditional Chinese Medicine, Chengdu, China

出　　处：《TMR Integrative Medicine》2019年第15期1-9,共9页TMR整合医学

摘　　要：Objective: To study on in immunological characteristics of T lymphocyte in peripheral blood from patients with Sjogren's syndrome (SS), provide the theory basis for the further research of Traditional Chinese medicine (TCM) targeted therapy.Methods:T lymphocyte subsets in peripheral blood from SS, RA and normal control groups were tested with various monocbnal antibodies and multicolor flow cytometry.T lymphocyte subsets were tested in peripheral blood from SS patients of different TCM Syndromes. The relationship between T Lymphocyte subsets and disease activity was measured by erythrocyte sedimentation rate (SR)and serum IgG. Results: The percentage of CD4^+T cells and the ration of CD4^+/CD8^+T cells in peripheral bbod from SS patients decreased significantly, and the percentage of CD8^+T cells increased than that in control groups (P<0.05). Compared with patients in inactive period, the percentage of CD4^+T cells in peripheral bLood from SS patients in the active stage increased (P>0.05), the percentage of CD8^+T cells decreased (P>0.05), and the ration of CD4^+/ CD8^+T cells increased (P<0.05).Compared with control groups, the percentage of CD69 expressed on T cells in peripheral blood from SS patients decreased, and the percentage of CD95's expression increased (P<0.05).The percentage of CD69CD95's expression also increased (P>0.05). There were no differences among CD4^+T cells, CD8^+T ceLs and CD4^+/ CD8^+ in peripheral blood from SS patients of different TCM Syndromes (P>0.05). CD69 and CD95's expression on SS patients of different TCM Syndromes was no significantLy different (P>0.05).T cell receptor Vβ(TCR Vβ) subfamilies gene in peripheral blood from SS patients and control groups were expressed in all. Compared witji control groups, Vβ 5.3,Vβ 13.1,Vβ 12,Vβ 7.2 had significant differences (P<0.05).Comparison of different TCM Syndromes in SS group: Vβ 13.1 and Vβ 14 were significantly different (P<0.05).Conclusion:There was dysimmunity on T lymphocyte subsets in peripheral blood of SS patients, wh

关 键 词：Sjogren's syndrome T lymphocyte SUBSETS FLow CYTOMETRY MONOCLONAL antibody T cell receptor VΒ gene 

分 类 号：R[医药卫生]