响应面法优化褶牡蛎多糖多肽联产工艺  

Optimization of Enzymatic Hydrolysis of Alectryonella plicatula Gmelin for Simultaneous Production of Polysaccharides and Polypeptides by Response Surface Methodology

作　　者：杨大俏 王锦旭[1,3] 李来好 杨贤庆[1] 马海霞[1] 岑剑伟[1] 王悦齐[1] YANG Daqiao;WANG Jinxu;LI Laihao;YANG Xianqing;MA Haixia;CEN Jianwei;WANG Yueqi(Key Laboratory of Aquatic Product Processing,Ministry of Agriculture and Rural Affairs,South China Sea Fisheries Research Institute,Chinese Academy of Fishery Science,Guangzhou 510300,China;College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306,China;School of Life Sciences and Food Technology,Hanshan Normal University,Chaozhou 521041,China)

机构地区：[1]中国水产科学研究院南海水产研究所,农业农村部水产品加工重点实验室,广东广州510300 [2]上海海洋大学食品学院,上海201306 [3]韩山师范学院食品工程与生物科技学院,广东潮州521041

出　　处：《食品科学》2019年第20期269-278,共10页Food Science

基　　金：中国水产科学研究院基本科研业务费资助项目（2019ZD1001）;广东省公益研究与能力建设专项（2017B020204001）;广东省自然科学基金项目（2016A030313144）;广东省促进经济高质量发展专项资金海洋经济发展项目(GDOE[2019]A25)

摘　　要：利用响应面法优化褶牡蛎(Alectryonella plicatula Gmelin)多糖多肽联产工艺.单因素试验研究酶添加量、酶解时间、料液比对多肽质量分数、总糖质量分数、水解度及酸性糖质量分数的影响,在此基础上,应用Design-Expert 8.0.5.0建立数学模型,进行3因素3水平的响应面分析.结果表明,酶解法联产制备褶牡蛎多糖多肽的最佳工艺为:添加0.2%的胰蛋白酶(≥250 U/mg),酶解80 min,料液比1:3(g/mL).此条件下进行重复实验,得到褶牡蛎酶解液中多肽质量分数为(85.14±1.67)%,总糖质量分数为(28.35±2.72)%,水解度为(11.12±0.61)%,酸性糖质量分数为(1.60±0.10)%.与回归方程预测值相近,说明褶牡蛎多糖多肽联产工艺可行.并按此工艺酶解褶牡蛎全脏器匀浆,粗多糖冻干品得率为(29.39±0.41)%;粗多肽冻干品得率为(36.81±0.47)%.研究为褶牡蛎活性多糖和多肽的联产制备提供了理论依据,可为企业创造更大的经济效益,以期实现褶牡蛎资源的综合利用.To simultaneously produce polysaccharides and polypeptides with a higher yield, the enzymatic hydrolysis of Alectryonella plicatula Gmelin was optimized using one-factor-at-a-time (OFAT) method and response surface methodology. Firstly, OFAT experiments were conducted to evaluate the effect of enzyme dosage, hydrolysis time and solid-to-water ratio on the concentrations of polypeptides, total sugar and glycosaminoglycan and the degree of hydrolysis. Then further experiments were designed by using Design-Expert 8.0.5.0 considering three independent variables each at three levels. Four mathematical models were built using the concentrations of polypeptides, total sugar and glycosaminoglycan and the degree of hydrolysis as responses, respectively. The results showed that the optimal conditions were found to be hydrolysis with 0.2% trypsase (≥ 250 U/mg) for 80 min at a solid-to-water ratio of 1:3 (g/mL). Experiments carried out under these conditions led to a polypeptide concentration of (85.14 ± 1.67)%, total sugar concentration of (28.35 ± 2.72)%, glycosaminoglycan concentration of (1.60 ± 0.10)%, and degree of hydrolysis of (11.12 ± 0.61)%. Good agreement between these values and the model predictions was found, suggesting that the optimized process was feasible. The yields of polypeptides and polysaccharides on a dry basis were (29.39 ± 0.41)% and (36.81 ± 0.47)%, respectively. This study provides a theoretical basis for simultaneous production of polysaccharides and polypeptides from A. plicatula, which will create greater economic benefits for enterprises by comprehensive utilization of A. plicatula.

关 键 词：褶牡蛎 多糖 多肽 联产工艺 

分 类 号：S985.35[农业科学—捕捞与储运]