Effect of chiropractic manipulation on disrupted epithelium barrier and its mechanism of specialized pro-resolving mediators in a spleen-deficiency murine model  

作　　者：Xiong Ying Song Zhixiu Gu Yun Qin Yuhang Wei Jiangfei Lu Jinlu Zhu Y 

机构地区：[1]Teaching and Research Section of Tuina,the Second Clinical Medical College,Nanjing University of Chinese Medicine, Nanjing 210023,China [2]Teaching and Research Section of Nutrition, the Second Clinical Medical College,Nanjing University of Chinese Medicine,Nanjing 210023,China [3]Laboratory of Acupuncture and Medicine Research of Ministry of Education,the Second Clinical Medical College,Nanjing University of Chinese Medicine,Nanjing 210023,China

出　　处：《Journal of Traditional Chinese Medicine》2019年第5期678-684,共7页中医杂志（英文版）

基　　金：Supported by the National Natural Science Foundationfunded Project:the Mechanism of Spine-pinching Manipulation via Inflammation Regulation of SPMs on Prevention and Treatment of Spleen-deficiency Asthma(No.81774446)

摘　　要：OBJECTIVE: To investigate the influence of spleen deficiency on the epithelial barrier of jejunum and lungs in a rat model of spleen-deficiency and the effect and potential specialized pro-resolving mediators (SPMs) mechanism of chiropractic manipulation. METHODS: Three-week-old male Sprague-Dawley rats were divided randomly into normal control group (n = 6), spleen-deficiency group (n = 5) and chiropractic group (n = 6). Spleen-deficiency model was induced in spleen-deficiency group and chiropractic group. Moreover, chiropractic manipulation was performed in chiropractic group. Four weeks later, systemic Th1/Th2 balance was evaluated by the ratio of plasma interferon (IFN)-γ/interleukin (IL)-4 levels by enzyme-linked immunosorbent assay (ELISA). Epithelial barrier integrity were assessed by the observation of morphological changes by hematoxylin-eosin staining and zonula occludens (ZO)-1 gene expressions by quantitative real time polymerase chain reaction in jejunum and lungs. Plasma resolvin D1 (RvD1) and lipoxin A4 (LXA4) levels were measures by ELISA for endogenous SPMs production. The levels of docosahexaenoic acid (DHA) and arachidonic acid (AA) in jejunum and lungs were also measured by HPLC-MS/ MS. RESULTS: Comparing with normal control group, spleen-deficiency group showed disrupted mucosa in jejunum, inflammatory condition in lungs, significantly decreased ratio of plasma IFN-γ/IL-4 levels and lower expressions of ZO-1 mRNA in both jejunum and lung tissues. Comparing with spleen-deficiency group, chiropractic group had less disrupted mucosa in jejunum and inflammatory condition in lungs, significantly increased systemic ratio of IFN-γ/IL-4 and expressions of ZO-1 mRNA in both jejunum and lung tissues. Chiropractic group had significantly enhanced plasma levels of RvD1 and LXA4, but had no significantly higher levels of DHA and AA in jejunum and lungs when comparing with spleen-deficiency group. CONCLUSION: Spleen deficiency caused systemic Th1/Th2 imbalance towards Th2 polarization and epithelial barOBJECTIVE: To investigate the influence of spleen deficiency on the epithelial barrier of jejunum and lungs in a rat model of spleen-deficiency and the effect and potential specialized pro-resolving mediators(SPMs) mechanism of chiropractic manipulation.METHODS: Three-week-old male Sprague-Dawley rats were divided randomly into normal control group(n = 6), spleen-deficiency group(n = 5) and chiropractic group(n = 6).Spleen-deficiency model was induced in spleen-deficiency group and chiropractic group.Moreover, chiropractic manipulation was performed in chiropractic group.Four weeks later, systemic Th1/Th2 balance was evaluated by the ratio of plasma interferon(IFN)-γ/interleukin(IL)-4 levels by enzyme-linked immunosorbent assay(ELISA).Epithelial barrier integrity were assessed by the observation of morphological changes by hematoxylin-eosin staining and zonula occludens(ZO)-1 gene expressions by quantitative real time polymerase chain reaction in jejunum and lungs.Plasma resolvin D1(RvD1) and lipoxin A4(LXA4) levels were measures by ELISA for endogenous SPMs production.The levels of docosahexaenoic acid(DHA) and arachidonic acid(AA) in jejunum and lungs were also measured by HPLC-MS/MS.RESULTS: Comparing with normal control group,spleen-deficiency group showed disrupted mucosa in jejunum, inflammatory condition in lungs, significantly decreased ratio of plasma IFN-γ/IL-4 levels and lower expressions of ZO-1 m RNA in both jejunum and lung tissues.Comparing with spleen-deficiency group, chiropractic group had less disrupted mucosa in jejunum and inflammatory condition in lungs, significantly increased systemic ratio of IFN-γ/IL-4 and expressions of ZO-1 m RNA in both jejunum and lung tissues.Chiropractic group had significantly enhanced plasma levels of RvD1 and LXA4, but had no significantly higher levels of DHA and AA in jejunum and lungs when comparing with spleen-deficiency group.CONCLUSION: Spleen deficiency caused systemic Th1/Th2 imbalance towards Th2 polarization and epithelial barrier disruption in jeju

关 键 词：Manipulation  CHIROPRACTIC Th1-Th2 balance Epithelial BARRIER Specialized pro-resolving MEDIATORS Spleen DEFICIENCY 

分 类 号：R[医药卫生]