Composition analysis and antioxidant activities of the Rhus typhina L.stem  

作　　者：Ting Liu Zhaoqin Li Ruiyun Li Yue Cui Yunli Zhao Zhiguo Yu 

机构地区：[1]School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenhe District, Shenyang 110016, China [2]The Precise Medicine Center, Key Laboratory of Environmental Pollution and Microecology, Liaoning Province,College of Basic Medical Sciences, Shenyang Medical College, No. 146, North Huanghe Street, Huanggu District, Shenyang 110034, China

出　　处：《Journal of Pharmaceutical Analysis》2019年第5期332-338,共7页药物分析学报（英文版）

摘　　要：The present investigation reports the chemical composition of the Rhus typhina L. stem identified via mass spectrometry and NMR as gallic acid, 1-O-galloyl-β-D-glucose, tryptophan, scopolin, methyl gallate, fustin, quercetin, rutin, and 1,2,3,4,6-penta-O-galloyl-β-D-glucose. The antioxidant properties and the chemical composition contents of the R. typhina L. stem grown in different regions in China were determined. To determine the antioxidant activity, a total phenolic content analysis, 2, 2-diphenyl-1-picrylhydrazyl radical scavenging activity assay, ferric reducing antioxidant power assay, and β-carotene linoleic acid model system were conducted. The results showed that the Rhus typhina L. stem possessed high antioxidant capacities due to its high phenolic content. The contents of the nine isolated compounds were determined by UPLC-ESI-MS/MS. The calibration curves of the nine isolated compounds were linear within the concentration range and the average recoveries were high. The result showed that 1-Ogalloyl-β-D-glucose, gallic acid, methyl gallate, and 1,2,3,4,6-penta-O-galloyl-β-D-glucose could be the compounds mainly responsible for the antioxidant capacity of the R. typhina L. stem. This reveals that the R. typhina L. stem is a good source of antioxidants.The present investigation reports the chemical composition of the Rhus typhina L.stem identified via mass spectrometry and NMR as gallic acid,1-O-galloyl-β-D-glucose,tryptophan,scopolin,methyl gallate,fustin,quercetin,rutin,and 1,2,3,4,6-penta-O-galloyl-β-D-glucose.The antioxidant properties and the chemical composition contents of the R.typhina L.stem grown in different regions in China were determined.To determine the antioxidant activity,a total phenolic content analysis,2,2-diphenyl-l-picrylhydrazyl radical scavenging activity assay,ferric reducing antioxidant power assay,andβ-carotene linoleic acid model system were conducted.The results showed that the Rhus typhina L.stem possessed high antioxidant capacities due to its high phenolic content.The contents of the nine isolated compounds were determined by UPLC-ESI-MS/MS.The calibration curves of the nine isolated compounds were linear within the concentration range and the average recoveries were high.The result showed that l-Ogalloyl-β-D-glucose,gallic acid,methyl gallate,and 1,2,3,4,6-penta-O-galloyl-β-D-glucose could be the compounds mainly responsible for the antioxidant capacity of the R.typhina L.stem.This reveals that the R.typhina L.stem is a good source of antioxidants.

关 键 词：RHUS typhina L. STEM ANTIOXIDANT ACTIVITY Chemical COMPOSITION UPLC-ESI-MS/MS 

分 类 号：R[医药卫生]