The effect of human amniotic epithelial cells on urethral stricture fibroblasts  

作　　者：Sanjay Gottipamula Sudarson Sundarrajan Kumar Chokalingam K.N.Sridhar 

机构地区：[1]Sri Research for Tissue Engineering Pvt.Ltd.,Shankara Research Centre [2]Cancyte Technologies Pvt.Ltd.,Rangadore Memorial Hospital,Bengaluru,Karnataka,India

出　　处：《Journal of Clinical & Translational Research》2019年第1期44-49,共6页临床和转化研究

摘　　要：Background:Urethral stricture disease(USD)is effectively managed by buccal mucosa(BM)urethroplasty.Lack of adequate healthy BM has led to the use of autologous tissue-engineered BM grafts.Such grafts are costly,not easily scalable and recurrence of the stricture is still a problem.Hence,there is a requirement for cost-effective,scalable cells with innate antifibrotic properties which seem to be fulfilled by human amniotic epithelial cells(HAMECs).The effect of HAMECs on USD is unknown.Aim:To study the effect of HAMECs-CM on human urethral stricture fibroblast(USF)cells by using in-vitro migration assay and molecular techniques.Materials and Methods:USF cells were derived from six patients undergoing urethroplasty.HAMECs were derived from one placenta after delivery.The effect of HAMECs-CM on USF cell migration was observed using a standard in vitro scratch assay over a period of 3 days.The effect of HAMECs-CM on the expression levels of markers alpha-smooth muscle actin(α-SMA)and tissue inhibitor of metalloproteinases(TIMP-1)in USF cells was also examined.Results:The HAMECs-CM suppressed the migration of USF cells in in vitro scratch assay.The HAMECs-CM consistently downregulatedα-SMA,but not TIMP-1.Conclusions:HAMECs have shown antifibrotic activity on USF cells in this in vitro study.Relevance for Patients:HAMECs could serve as an alternative cell source for tissue-engineered urethroplasty.

关 键 词：Alpha-smooth muscle ACTIN Amniotic EPITHELIAL cells Fibrosis Tissue inhibitor of metalloproteinases-1 URETHRAL STRICTURE 

分 类 号：R73[医药卫生—肿瘤]