双水相技术提取黄秋葵黄酮的工艺条件研究  

Study on extraction of flavonoids from okra by aqueous two-phase technology

作　　者：董彩文[1,2] 王浩瑾 张明俊 席红如 DONG Cai-wen;WANG Hao-jing;ZHANG Ming-jun;XI Hong-ru(School of Food and Biological Engineering,Zhengzhou University of Light Industry,Zhengzhou 450002,Henan,China;Henan Provincial Collaborative Innovation Center of Food Production and Safety,Zhengzhou 450002,Henan,China;Affiliated Hospital of Zhengzhou University of Light Industry,Zhengzhou 450002,Henan,China)

机构地区：[1]郑州轻工业学院食品与生物工程学院,河南郑州450002 [2]河南省食品生产与安全协同创新中心,河南郑州450002 [3]郑州轻工业学院校医院,河南郑州450002

出　　处：《粮食与油脂》2019年第10期85-88,共4页Cereals & Oils

基　　金：食品生产与安全河南省协同创新中心研究生科技创新基金

摘　　要：研究乙醇-硫酸铵双水相体系提取黄秋葵中黄酮的最佳提取条件,通过单因素试验和响应面分析方法对黄酮的提取条件进行选择和优化。得到的最优结果:无水乙醇的体积分数为35%,提取时间为9.5 min,提取温度为40.5℃,在此条件下进行试验,得到黄酮的提取率为83.09%,实际测定值与预测值的相对误差为1.3%,表明该方法是可靠的。The optimum extraction conditions of flavonoids from okra by ethanol-ammonium sulfate aqueous two-phase system were studied.The flavonoids extraction conditions were selected and optimized through single factor experiment and response surface analysis method.The optimum results obtained were as follows:ethanol volume fraction 35%,extraction time 9.5 min,extraction temperature 40.5℃,and the extraction yeild of flavonoids was 83.09%.The relative error between actual value and predicted value was 1.3%,indicating that the parameter was reliable.

关 键 词：黄秋葵 双水相萃取 黄酮 

分 类 号：TS201.1[轻工技术与工程—食品科学]