分蘖洋葱冰结构蛋白的提取及鉴定  

Extraction and Identification of Ice Structural Protein from Allium cepa

作　　者：田野[1] 李亚楠 吴征 王紫薇 陈凤莲[1] 曲敏[1] TIAN Ye;LI Ya-nan;WU Zheng;WANG Zi-wei;CHEN Feng-lian;QU Min(Key Laboratory of Food Science and Engineering in Colleges and Universities of Heilongjiang Province,College of Food Engineering,Harbin University of Commerce,Harbin 150076,China)

机构地区：[1]哈尔滨商业大学食品工程学院黑龙江省普通高校食品科学与工程重点实验室

出　　处：《中国调味品》2019年第11期44-50,共7页China Condiment

基　　金：哈尔滨商业大学科学基金项目(18XN073)

摘　　要：以耐寒性较好的东北分蘖洋葱为原料,利用冰结合磷酸缓冲溶液法,提取分蘖洋葱冰结构蛋白。采用单因素和响应曲面法,考察了磷酸缓冲液(PBS)的浓度、料液比及冰球数量对冰结构蛋白提取率的影响。确定最佳参数为:分蘖洋葱滤汁与PBS溶液的体积比为1∶5.32,PBS浓度为91.87 mmol/L,冰球添加量为33个/100 mL。在此条件下,AISPs的提取率达到65.23%。经全波长紫外扫描,在280 nm处有最大吸收峰。利用SDS-PAGE确定其分子量为52,29,22,15 kDa。将AISPs添加到耐低糖酵母菌悬液中,当添加量为1.0%、冻融周期为5 d/次时,酵母菌的活菌数最高,菌落数为6.73×10^5个,存活率为51.45%,分别比对照组提高了88.5%和25.56%,说明AISPs具有很好的抗冻保护活性。In this experiment,the ice structural protein of Allium cepa is extracted by ice combined with phosphoric acid buffer solution method,using the northeastern Allium cepa with better cold resistance as the raw material.The effects of the concentration of phosphate buffer solution(PBS),the ratio of material to liquid and the number of ice balls on the extraction rate of ice structural protein(ISP)are investigated by single factor and response surface methodology(RSM).The optimum parameters are determined as follows:the volume ratio of Allium cepa juice to PBS is 1∶5.32,the concentration of PBS is 91.87 mmol/L,and the additive amount of ice balls is 33/100 mL.Under these conditions,the extraction rate of AISPs reaches 65.23%.The maximum absorption peak is found at 280 nm by full wavelength ultraviolet scanning.Its molecular weight is determined by SDS-PAGE as 52,29,22,15 kDa.When AISPs are added to low sugar-resistance yeast suspension,when the additive amount is 1.0%and the freezing-thawing period is 5 d/time,the number of viable yeasts is the highest,the number of colonies is 6.73×105,and the survival rate is 51.45%.Compared with the control group,the survival rate is increased by 88.5%and 25.56%respectively,which indicates that AISPs have good antifreeze protection activity.

关 键 词：分蘖洋葱 冰结构蛋白 冰提 响应曲面 酵母抗冻保护 

分 类 号：TS201.3[轻工技术与工程—食品科学]