Comprehensive transcriptome analysis based on RNA sequencing identifies critical genes for lipopolysaccharide-induced epididymitis in a rat model  

作　　者：Xin Song Nan-He Lin You-Lin Wang Bin Chen Hong-Xiang Wang Kai Hu 

机构地区：[1]Department of Urology,Shanghai Institute of Andrology,Renji Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200001,China.

出　　处：《Asian Journal of Andrology》2019年第6期605-611,共7页亚洲男性学杂志（英文版）

基　　金：This study was funded by the National Natural Science Foundation of China(No.81471496).

摘　　要：Epididymitis is a comm only diagnosed disease associated with male infertility.However,little is known about the molecules that are involved in its development.This study was to identify critical genes associated with lipopolysaccharide-induced epididymitis and an a lyze the molecular mecha nism of epididymitis through RNA sequencing.Experime ntal epididymitis models were generated by administering male Sprague-Dawley rats'lipopolysaccharide.A total of 1378 differentially expressed genes,including 531 upregulated and 847 down regulated gen es,were identified in the epididymitis model rats compared with those in sham-operated rats by RNA seque ncing.Fun ctional enrichme nt an a lyses suggested that the upregulated genes were markedly enriched in inflammati on.related biological processes,as well as in the tumor necrosis factor(TNF)signaling pathway,cytokine-cytokine receptor interactions,complement and coagulation cascades,and in the chemokine signalipathway.Four downregulated genes(collagen type XXVⅢalpha 1 chain[Col28α1],cyclirndependent kinase-like 1[Cdkl1],phosphoserine phosphatase[Psph],and fatty acid desaturase 2[Fads2])and ten upregulated genes(CCAAT/enhancer-binding protein beta[Cebp0],C-X-C motif chemokine receptor 2[Cxcr2],interleukin 11[ll11],C-C motif chemokine ligand 20[Ccl20],nuclear factor-kappa-B inhibitor alpha[NfKbia],claudin 4[Cldn4],matrix metallopeptidase 9[Mmp9],heat shock 70 kDa protein 8[Hspa8],intercellular cell adhesion molecule-1[Icam1],and Jun)were successfully confirmed by real-time polymerase chai n reaction.Wester n blot dem on strated that CDKL1 was decreased,while MMP9 and NFKBIA were increased in the experimental model group compared with those in the sham-operated group.Our study sheds new light on the understand!ng of the early response of the epididymis during bacterial epididymitis.

关 键 词：DIFFERENTIALLY EXPRESSED GENES EPIDIDYMITIS INFLAMMATION LIPOPOLYSACCHARIDE 

分 类 号：R73[医药卫生—肿瘤]