转谷氨酰胺酶的固定化及其在处理米糠废水中的应用  

Immobilization of Transglutaminase and Its Application in the Treatment of Rice Bran Wastewater

作　　者：裴兴武 汪鸿 高子雯 袁泰增 高明[1] 姚凯 潘明喆[1] 于殿宇[1] PEI Xingwu;WANG Hong;GAO Ziwen;YUAN Taizeng;GAO Ming;YAO Kai;PAN Mingzhe;YU Dianyu(College of Food Science,Northeast Agricultural University,Harbin 150030,China;Hubei Tianxing Grain and Oil Co.Ltd.,Suizhou 441300,China)

机构地区：[1]东北农业大学食品学院,黑龙江哈尔滨150030 [2]湖北天星粮油股份有限公司,湖北随州441300

出　　处：《食品科学》2019年第22期110-117,共8页Food Science

基　　金：“十三五”国家重点研发计划重点专项(2018YFD0401101)

摘　　要：制备醋酸纤维素-聚丙烯复合膜,并将转谷氨酰胺酶(transglutaminase,MTG)固定在膜上,制得了固定化MTG酶膜,测得其酶活为17.6 U/g。然后将酶膜固定在不锈钢网框上,并将其悬挂在烧杯中,用来处理米糠废水中的蛋白质。利用单因素试验,分别考察物料温度、物料pH值、转子转速以及酶膜面积对蛋白质聚合率的影响,并在单因素试验基础上,利用响应面试验优化,得到最佳聚合条件为物料温度43℃、pH 6.6、转子转速124 r/min、酶膜面积与底物含量比例为80∶1(cm^2/g)、反应时间1.8 h,蛋白质聚合率为70%。固定化酶膜在使用5次后,相对酶活力仍保持在74.3%以上,为连续聚合米糠废水中的蛋白质以及保留营养物质提供理论基础。In this study,cellulose acetate-polypropylene composite membrane was prepared,and microbial transglutaminase(MTG)was immobilized onto the membrane,yielding an MTG-immobilized membrane,whose enzymatic activity was determined to be 17.6 U/g.Then the enzyme-loaded membrane was fixed on a stainless steel mesh frame and suspended in a beaker in order to use it to treat protein in rice bran wastewater.The effects of material temperature,material pH,rotor speed and enzyme-loaded membrane area on protein polymerization rate were investigated by one-factor-at-a-time method.Furthermore,using response surface optimization,the optimum polymerization conditions were obtained as follows:material temperature 43℃,pH 6.6,rotor speed 124 r/min,ratio between enzyme-loaded membrane area and substrate amount 80:1(cm2/g),and reaction time 1.8 h.Under these conditions,protein polymerization rate was 70%.The enzymeimmobilized membrane remained above 74.3%of the original activity after its fifth repeated use.This study provides a theoretical basis for the continuous polymerization of proteins in rice bran wastewater and the retention of nutrients.

关 键 词：转谷氨酰胺酶 复合酶膜 米糠废水 蛋白质聚合率 重复使用 

分 类 号：TS213.3[轻工技术与工程—粮食、油脂及植物蛋白工程]