一株产吲哚乙酸耐盐促生菌的分离、鉴定及发酵条件优化  

Isolation, identification and fermentation conditions optimization of a salt-tolerant,growth-promoting and indoleacetic acid-producing bacterium

作　　者：贾西贝 王琦琦 李杨[1] 褚贵新 孙燕飞[1] JIA Xibei;WANG Qiqi;LI Yang;CHU Guixin;SUN Yanfei(College of Life Sciences,Shihezi University,Shihezi 832003,China;Department of Environmental Science and Engineering,Shaoxing University,Shaoxing 312000,China)

机构地区：[1]石河子大学生命科学学院,新疆石河子832003 [2]绍兴文理学院环境科学与工程系,浙江绍兴312000

出　　处：《中国酿造》2019年第11期37-42,共6页China Brewing

基　　金：国家重大基础研究专项新疆干旱区盐碱地生态治理关键技术研究与集成示范(2016YFC0501404)

摘　　要：从碱蓬根际土壤中分离筛选获得一株能产吲哚乙酸(IAA)的促生菌株BG-5,对该菌株进行了16S rDNA序列分析。以IAA的产量作为评价指标,利用单因素试验及响应面分析法对发酵条件及培养基成分进行优化。结果表明,BG-5菌株被鉴定为短小芽孢杆菌(Bacillus pumilus),最佳培养条件为装液量30 mL/250 mL、初始pH值为8、培养温度37℃;优化后的培养基成分为酵母提取物7.3 g/L,胰蛋白胨8.8 g/L,七水硫酸镁7.2 g/L;在此最优条件下,BG-5菌株的吲哚乙酸产量为87.86μg/mL,约为优化前的2倍。A growth-promoting and indoleacetic acid(IAA)-producing strain BG-5 was isolated and screened from the rhizosphere soil of Suaeda salsa,and analyzed by 16S rDNA sequences.Using the yield of IAA as evaluation index,the fermentation conditions and medium composition were optimized by single factor tests and response surface methodology.The results showed that the strain BG-5 was identified as Bacillus pumilus.The optimal culture conditions were loading volume 30 ml/250 ml,initial pH 8 and culture temperature 37℃.The optimal medium composition was yeast extract 7.3 g/L,tryptone 8.8 g/L,and magnesium sulfate heptahydrate 7.2 g/L.Under the optimal conditions,the IAA yield of the strain BG-5 was 87.86μg/ml,which was about twice that of before optimization.

关 键 词：菌株筛选 鉴定 吲哚乙酸 培养基成分 发酵条件 优化 

分 类 号：S154.3[农业科学—土壤学]