Systems-Based Interactome Analysis for Hematopoiesis Effect of Angelicae sinensis Radix: Regulated Network of Cell Proliferation towards Hemopoiesis  

作　　者：ZHENG Guang ZHANG He YANG Yun SUN Ying-li ZHANG Yan-jing CHEN Ju-ping HAO Ting LU Cheng GUO Hong-tao ZHANG Ge FAN Dan-ping HE Xiao-juan LU Ai-ping 

机构地区：[1]Information Science and Engineering School,Lanzhou University,Lanzhou(730000),China [2]School of Biology,Lanzhou University,Lanzhou(730000),China [3]Institute of Basic Research in Chinese Medicine,China Academy of Chinese Medical Sciences,Beijing(100700),China [4]Department of Rheumatism,the First Affiliated Hospital of Henan University of Traditional Chinese Medicine,Zhengzhou(450000),China [5]School of Chinese Medicine,Hong Kong Baptist University,Kowloon Tong,Kowloon,Hong Kong SAR,China

出　　处：《Chinese Journal of Integrative Medicine》2019年第12期939-947,共9页中国结合医学杂志（英文版）

摘　　要：Objective: To explore the molecular-level mechanism on the hematopoiesis effect of Angelicae sinensis Radix(ASR) with systems-based interactome analysis. Methods: This systems-based interactome analysis was designed to enforce the workflow of "ASR(herb)→compound→target protein→internal protein actions→ending regulated protein for hematopoiesis". This workflow was deployed with restrictions on regulated proteins expresses in bone marrow and anemia disease and futher validated with experiments. Results: The hematopoiesis mechanism of ASR might be accomplished through regulating pathways of cell proliferation towards hemopoiesis with cross-talking agents of spleen tyrosine kinase(SYK), Janus kinase 2(JAK2), and interleukin-2-inducible T-cell kinase(ITK). The hematopoietic function of ASR was also validated by colonyforming assay performed on mice bone marrow cells. As a result, SYK, JAK2 and ITK were activated. Conclusion: This study provides a new approach to systematically study and predict the therapeutic mechanism for ASR based on interactome analysis towards biological process with experimental validations.Objective: To explore the molecular-level mechanism on the hematopoiesis effect of Angelicae sinensis Radix(ASR) with systems-based interactome analysis. Methods: This systems-based interactome analysis was designed to enforce the workflow of "ASR(herb)→compound→target protein→internal protein actions→ending regulated protein for hematopoiesis". This workflow was deployed with restrictions on regulated proteins expresses in bone marrow and anemia disease and futher validated with experiments. Results: The hematopoiesis mechanism of ASR might be accomplished through regulating pathways of cell proliferation towards hemopoiesis with cross-talking agents of spleen tyrosine kinase(SYK), Janus kinase 2(JAK2), and interleukin-2-inducible T-cell kinase(ITK). The hematopoietic function of ASR was also validated by colonyforming assay performed on mice bone marrow cells. As a result, SYK, JAK2 and ITK were activated. Conclusion: This study provides a new approach to systematically study and predict the therapeutic mechanism for ASR based on interactome analysis towards biological process with experimental validations.

关 键 词：Angelicae SINENSIS RADIX INTERACTOME cell proliferation HEMOPOIESIS regulation cross-talking agents 

分 类 号：R73[医药卫生—肿瘤]