1株阿魏酸酯酶产生菌的筛选鉴定及发酵条件优化  

Screening and Identification of a Ferulic Acid Esterase Producing Strain and the Optimization of Fermentation Conditions

作　　者：曹艳[1] 夏其乐[1] 陈剑兵[1] 陆胜民[1] CAO Yan;XIA Qi-le;CHEN Jian-bing;LU Sheng-min(Inst.of Food Sci.,Zhejiang Acad.of Agric.Sci.,Key Lab.of Post-Harvest Handling of Fruits,Minist.of Agric.&Rural Affairs,Key Lab.of Fruits&Vegetables Postharvest&Processing Technol.Res.,Hangzhou 310021)

机构地区：[1]浙江省农业科学院食品科学研究所农业农村部果品采后处理重点实验室浙江省果蔬保鲜与加工技术研究重点实验室

出　　处：《微生物学杂志》2019年第5期8-15,共8页Journal of Microbiology

基　　金：国家重点研发计划项目(2018YFF0213500);浙江省农业科学院青年人才项目(10102000617CF0101G/003/010)

摘　　要：从浙江绍兴鉴湖附近土样中分离筛选到1株产阿魏酸酯酶(ferulic acid esterase,FAE)的菌株FD-8,通过菌落形态、分生孢子梗形态对比和ITS/18S rDNA分子生物学同源性分析,鉴定该菌株为溜曲霉,命名为Aspergillus tamarii FD-8。FD-8生长最适碳源和氮源分别为蔗糖和酵母浸提物,最适生长温度和pH分别为32℃和5.0,最适产酶条件为32℃、pH 6.0,发酵周期为120 h。在最适培养基中、分阶段控制pH培养条件下,FAE比酶活达到231.34 mU/mg。去淀粉麸皮可诱导FAE的合成,在发酵48 h添加20.0 g/L去淀粉麸皮,FAE最高比酶活可达到573.61 mU/mg,比对照提高1.48倍。A strain FD-8 producing ferulic acid esterase(FAE)with higher activity was isolated and screened from soil samples of Jianhu Lake in Shaoxing City of Zhejiang Province.It was identified as Aspergillus tamarii and named as A.tamarii FD-8 by comparing its morphology of colony and conidiophore and analyzing the molecular biology homology based on ITS/18S rDNA.The optimum carbon and nitrogen sources for the growth were sucrose and yeast extract,the optimum temperature and pH for the growth were 32℃and 5.0 respectively,and the optimum FAE production conditions were 32℃and pH 6.0 for 120 h of fermentation period.The specific activity of FAE was 231.34 mU/mg after optimization of fermentation conditions.In addition,the synthesis of FAE could be induced by discarded starch wheat bran(DSWB)during the fermentation of FD-8 at 48 h and adding 20.0 g/L pf DSWB.The maximum specific activity of FAE could reach up to 573.61 mU/mg increased by 1.48 folds as compared with the control one.

关 键 词：阿魏酸酯酶 菌种筛选 鉴定 发酵条件优化 

分 类 号：Q93-331[生物学—微生物学]