水发产品中甲醛快速检测方法的比较研究  

Research on rapid detection of formaldehyde in hydrated products

作　　者：聂小林 汪开银 游京晶[2] 李淑慧 王倩 熊晓辉[2] NIE Xiao-Lin;WANG Kai-Yin;YOU Jing-Jing;LI Shu-Hui;WANG Qian;XIONG Xiao-Hui(National Light Industry Food Quality Supervision and Inspection in NanJing,NanJing 211800,China;College of Food and Light Industry,Nanjing Tech University,NanJing 211800,China)

机构地区：[1]国家轻工业食品质量监督检测南京站,南京211800 [2]南京工业大学食品与轻工学院,南京211800

出　　处：《食品安全质量检测学报》2019年第21期7301-7310,共10页Journal of Food Safety and Quality

基　　金：国家市场监督管理总局研究项目~~

摘　　要：目的 比较快速检测水发产品中甲醛的方法 .方法本研究以4-氨基-3-联氨-5-巯基-1,2,4-三氮杂茂(4-amino-3-hydrazine-5-mercapto-1,2,4-triazo,AHMT)法(比色卡法)、AHMT法(分光光度法)和乙酰丙酮法(分光光度法)作为水发产品中甲醛的快速检测手段.试样中的甲醛经提取后,在碱性条件下与AHMT反应,再被高碘酸钾氧化成紫红色络合物或在沸水浴条件下与乙酰丙酮反应,生成黄色物质.通过比色进而建立的快速检测甲醛含量的方法.结果 经方法学验证表明,以竹笋、牛肚、银鱼作为空白基质的检测限为5 mg/kg,3种方法的灵敏度均≥95%、特异性均≥85%、假阴性率均≤5%和假阳性率均≤15%.结论 该方法快速准确,灵敏度高,可适用于水发产品中甲醛的快速测定.Objectvie To compare some rapid methods to detect formaldehyde in hydrated products. Methods This study used the 4-Amino-3-hydrazine-5-mercapto-1, 2, 4-triazo(AHMT) method(colorboard method), the AHMT method(spectrophotometric method) and the acetylacetone method(spectrophotometric method) as rapid detection methods for formaldehyde in hydrated products. After the formaldehyde in the sample was extracted, it reacted with the AHMT under alkaline conditions, and was then oxidized by potassium periodate to a purple-red complex or reacted with acetylacetone in a boiling water bath to produce a yellow substance. A method for rapid detection of formaldehyde content was established by colorimetry. Results Verification showed when used bamboo shoots, tripe and silverfish as blank matrix, the detection limit was 5 mg/kg. The sensitivity of the three methods≥95%, the specificity≥85%, the false negative rate≤5%, and the false positive rate≤5%. Conclusion The method is rapid, accurate and sensitive, suitable for the rapid detection of formaldehyde in hydrated products.

关 键 词：甲醛 水发产品 方法学验证 快速检测 

分 类 号：O65[理学—分析化学]