Developments in cell culture systems for human pluripotent stem cells  

作　　者：Weiwei Liu Chunhao Deng Carlos Godoy-Parejo Yumeng Zhang Guokai Chen 

机构地区：[1]Centre of Reproduction,Development and Aging,Faculty of Health Sciences,University of Macao,Macao 999078,China [2]Institute of Translational Medicine,Faculty of Health Sciences,University of Macao,Macao 999078,China [3]Bioimaging and Stem Cell Core Facility,Faculty of Health Sciences,University of Macao,Macao 999078,China

出　　处：《World Journal of Stem Cells》2019年第11期968-981,共14页世界干细胞杂志（英文版）（电子版）

基　　金：Supported by University of Macao Multi-Year Research Grants,No.MYRG2015-00228-FHS and MYRG2018-00135-FHS;Macao Science and Technology Development Fund,No.FDCT/131/2014/A3 and FDCT/056/2015/A2

摘　　要：Human pluripotent stem cells(hPSCs)are important resources for cell-based therapies and pharmaceutical applications.In order to realize the potential of hPSCs,it is critical to develop suitable technologies required for specific applications.Most hPSC technologies depend on cell culture,and are critically influenced by culture medium composition,extracellular matrices,handling methods,and culture platforms.This review summarizes the major technological advances in hPSC culture,and highlights the opportunities and challenges in future therapeutic applications.Human pluripotent stem cells(h PSCs) are important resources for cell-based therapies and pharmaceutical applications. In order to realize the potential of h PSCs, it is critical to develop suitable technologies required for specific applications. Most h PSC technologies depend on cell culture, and are critically influenced by culture medium composition, extracellular matrices, handling methods, and culture platforms. This review summarizes the major technological advances in h PSC culture, and highlights the opportunities and challenges in future therapeutic applications.

关 键 词：HUMAN pluripotent STEM CELLS HUMAN embryonic STEM CELLS CELL CULTURE CULTURE medium STEM CELL niche Signal transduction EMBRYOID bodies 

分 类 号：R73[医药卫生—肿瘤]