华山松籽油亚油酸的富集纯化及降血脂活性研究  

Enrichment of linoleic acid from Pinus armandi franch seed oil and its lipid-lowering activity

作　　者：郭剑霞 张谨华[1] 潘玉峰[1] 温学[1] GUO Jianxia;ZHANG Jinhua;PAN Yufeng;WEN Xue(College of Biological Science and Technology,Jinzhong University,Jinzhong 030619,Shanxi,China)

机构地区：[1]晋中学院生物科学与技术学院

出　　处：《中国油脂》2019年第11期131-136,共6页China Oils and Fats

基　　金：国家高技术研究发展计划“863计划”资助项目(2007AA100404);天津市高等学校科技发展基金计划项目(20110614);国家自然科学基金(81502963)

摘　　要：以华山松籽油为原料,通过皂化酸解得到混合脂肪酸,再通过尿素包合法富集纯化亚油酸,研究了包合温度、包合时间、混合脂肪酸与尿素质量比值、95%乙醇与尿素体积质量比值4个因素对亚油酸纯度和得率的影响。然后采用不同剂量的纯化亚油酸对高脂模型小鼠进行灌胃,研究其降血脂功能。通过单因素试验确定富集纯化亚油酸的最佳工艺条件为:包合温度-10℃,包合时间24 h,混合脂肪酸与尿素质量比值0.4,95%乙醇与尿素体积质量比值5。在最佳条件下,经一次尿素包合,得到的亚油酸纯度为80.57%。降血脂功能研究结果表明:由华山松籽油富集纯化得到的亚油酸具有降低高脂血症小鼠体重、肝脏重及肝脏指数、小鼠血清甘油三酯(TG)及总胆固醇(TC)水平,提高小鼠血清高密度脂蛋白胆固醇(HDL-C)水平,降低动脉硬化指数(AI)及低密度脂蛋白胆固醇(LDL-C)水平的功能。由华山松籽油富集纯化得到的亚油酸可降低高脂血症小鼠血脂水平,改善其因摄入过多脂质而导致的肝脏脂肪堆积和动脉粥样硬化。Pinus armandi franch seed oil was saponified and acidified to obtain the mixed fatty acid,and then the linoleic acid was enriched by urea adduction fractionation.The effects of adduction temperature,adduction time, mass ratio value of mixed fatty acid to urea, ratio value of 95% ethanol volume to mixed fatty acid mass on purity and yield of linoleic acid were studied.Different dosage of linoleic acid obtained was administered to the hyperlipidemic mice to evaluate its lipid-lowering function.The optimal enrichment conditions of linoleic acid were determined by single factor experiment as follows:adduction temperature-10℃, adduction time 24 h, mass ratio value of mixed fatty acid to urea 0.4, ratio value of 95% ethanol volume to urea mass 5.Under these conditions, the purity of linoleic acid was 80.57% after once urea adduction. The results of lipid-lowering evaluation showed that the linoleic acid from Pinus armandi franch seed oil could reduce the body weight, liver weight, liver index, serum total cholesterol(TC) and triglyceride(TG) levels of the hyperlipidemic mice. It could improve the high density lipoprotein cholesterol(HDL-C) level, reduce the low density lipoprotein cholesterol(LDL-C) level and arteriosclerosis index(AI) in mice. The linoleic acid obtained from Pinus armandi franch seed oil could reduce the blood lipid level of hyperlipidemic mice and improve the accumulation of liver fat and atherosclerosis caused by excessive intake of lipids.

关 键 词：华山松籽油 亚油酸 尿素包合 富集纯化 降血脂活性 

分 类 号：TS225.1[轻工技术与工程—粮食、油脂及植物蛋白工程] TS201.2[轻工技术与工程—食品科学与工程]