昆明市售花生酱和芝麻酱的菌相初步分析  

Bacterial community of peanut butter and sesame paste in Kunming market

作　　者：汤晓召 杨祖顺[1] 何玉凤 任翔 范璐[1] 国译丹[1] 杨菁 邹颜秋硕 田云屏[1] TANG Xiao-Zhao;YANG Zu-Shun;HE Yu-Feng;REN Xiang;FAN Lu;GUO Yi-Dan;YANG Jing;ZOU Yan-Qiu-Shuo;TIAN Yun-Ping(Yunan Center for Disease Control and Prevention,Kunming 650022,China)

机构地区：[1]云南省疾病预防控制中心

出　　处：《食品安全质量检测学报》2019年第22期7665-7668,共4页Journal of Food Safety and Quality

摘　　要：目的了解昆明市售花生、芝麻酱的菌相组成情况及其微生物污染风险。方法将16份昆明市售花生、芝麻酱进行增菌培养后,取增菌液进行D N A的提取,然后进行基于16S rRNA的宏基因组测序和分析。结果细菌主要来自于厚壁菌门和变形菌门,共检测出了40个细菌的属,其中包含多个含有致病菌的属。农贸市场采集样品中检测到的细菌属种类显著高于超市采集的样品。结论昆明市售花生、芝麻酱存在一定的微生物污染风险,农贸市场所售该类产品较超市产品存在较大污染风险,应引起重视。Objective To understand the bacterial phase composition and the risk of microbial contamination ofpeanuts and sesame paste sold in Kunming. Methods Sixteen samples of peanuts and sesame paste sold inKunming city were cultured for bacteria enrichment, and then DNA was extracted from the bacteria solution, and thenmacro genome sequencing and analysis were performed based on 16S rRNA. Results Bacteria were mainly fromFirmicutes and Proteobacteria, and a total of 40 genera of bacteria were detected, including several genera containingpathogenic bacteria. The bacteria species detected in the samples collected from farmers’ markets were significantlyhigher than those collected from supermarkets. Conclusion Peanut butter and sesame paste in Kunming marketexist some risk of contamination from microbial. Especially, we should pay more attention on the peanut butter andsesame paste from farmers? market, those have a greater risk of pollution than those sold in the supermarket.

关 键 词：花生酱 芝麻酱 菌相 宏基因组 

分 类 号：TS2[轻工技术与工程—食品科学与工程]