松仁清蛋白抗氧化肽的分离纯化及结构鉴定  

Isolation, Purification and Structural Identification of Antioxidant Peptides Derived from Enzymatic Hydrolysis of Pine Nut Kernel(Pinus koraiensis Sieb. et Zucc.) Albumin

作　　者：卢红妍 杨行 方丽[1,2] 刘诗梦 闵伟红 LU Hongyan;YANG Hang;FANG Li;LIU Shimeng;MIN Weihong(College of Food Science and Engineering,Jilin Agricultural University,Changchun 130118,China;National Engineering Laboratory on Wheat and Corn Further Processing,Changchun 130118,China)

机构地区：[1]吉林农业大学食品科学与工程学院,吉林长春130118 [2]小麦和玉米深加工国家工程实验室,吉林长春130118

出　　处：《食品科学》2019年第24期40-45,共6页Food Science

基　　金：国家高技术研究发展计划（863计划）项目（2013AA102206）;吉林省教育厅“十三五”科学技术项目（JJKH20170300KJ）

摘　　要：利用碱性蛋白酶酶解松仁清蛋白制备高抗氧化活性肽,通过超滤、SephadexG-25、SephadexG-15及反相高效液相色谱对抗氧化肽进行分离纯化,并采用电喷雾串联质谱进行结构鉴定。经筛选获得高抗氧化活性肽苯丙氨酸-苯丙氨酸-脯氨酸-酪氨酸(FFPY)和酪氨酸-亮氨酸-脯氨酸-苯丙氨酸(YLPF),分子质量分别为572.67 Da和538.65 Da。对FFPY和YLPF进行固相合成,纯度达到99.16%和99.91%,氧自由基吸收能力分别为7 329.63 μmol/g和2 835.47 μmol/g。Two peptides with strong antioxidant activity were seprate and purified from the alkaline protease of pine nut kernel albumin hydrolysate by by ultrafiltration, column chromatographies on Sephadex G-25, and Sephadex G-15 and reversed-phase high-performance liquid chromatography, and their structure was identified by electrospray ionization tandem mass spectrometry (ESI-MS/MS). The peptides were found to be Phe-Phe-Pro-Tyr (FFPY) and Tyr-Leu-Pro-Phe (YLPF) with molecular masses were 572.67 and 538.65 Da, respectively. Solid phase synthesis of FFPY and YLPF was performed with a purity of 99.16% and 99.91%. It was proved that the oxygen radical absorbance capacity (ORAC) values of FFPY and YLPF were 7 329.63 and 2 835.47 μmol/g, respectively.

关 键 词：松仁清蛋白 抗氧化肽 分离纯化 结构鉴定 

分 类 号：TS209[轻工技术与工程—食品科学]