Oxyresveratrol suppresses proliferation and metastasis of hepatocellular carcinoma by inhibiting autophagy in vitro and in vivo  

作　　者：SONG Ying-qi WU Xi-cheng LI Rong JI Zhen-ni MA Xiu-ying XIONG Wen-bi ZHANG Yuan-yuan ZHOU Li-ming 

机构地区：[1]Department of Pharmacology,West China School of Basic Medical Sciences and Forensic Medicine,Sichuan University,Chengdu 610041,China [2]Basic Medicine 2015 Undergraduate Students,West China School of Basic Medical Sciences and Forensic Medicine,Sichuan University,Chengdu 610041,China [3]State Key Lab of Biotherapy and Cancer Center,West China Hospital,Sichuan University and Collaborative Innovation Center for Biotherapy,Chengdu 610041,China

出　　处：《中国药理学与毒理学杂志》2019年第9期695-696,共2页Chinese Journal of Pharmacology and Toxicology

摘　　要：OBJECTIVE Oxyresveratrol(OXY)has many biological activities including anti-inflammation,anti-oxida⁃tive stress,anti-cancer and immunomodulation.However,the mechanisms underlying its effects against hepatocellular carcinoma(HCC)remain poorly understood.METHODS Two HCC cell lines,HepG2 and SMMC-7721 cells,were employed.Their proliferation was determined by CCK8 assay.The migration and invasion of cells were examined by wound healing and transwell assay.Metastasis of HCC was detected by in vivo experiment.Meanwhile,transcriptome analysis was applied to explore the mechanisms of OXY.The results of transcriptome analysis were validated by in vitro experiment.Further⁃more,western blot was used to measure the expression of LC3 and p62 protein.RESULTS OXY significantly inhibited the proliferation,migration and invasion of HCC cells in vitro.OXY suppressed the metastasis of HCC in Balb/c mice with attenuated side effects compared to Doxorubicin.Transcription profiling analysis revealed that OXY may affect autophagy related signaling pathway of HepG2 cells.Western blotting showed that OXY significantly inhibite autophagy by downregulating LC3 and upregulating p62 genes expression.CONCLUSION Our study demonstrated that OXY inhibits the metastasis of HCC by inhibiting autophagy,which suggested OXY to be a candidate for HCC metastasis.OBJECTIVE Oxyresveratrol(OXY) has many biological activities including anti-inflammation, anti-oxidative stress, anti-cancer and immunomodulation. However, the mechanisms underlying its effects against hepatocellular carcinoma(HCC) remain poorly understood. METHODS Two HCC cel lines, HepG 2 and SMMC-7721 cel s, were employed.Their proliferation was determined by CCK8 assay. The migration and invasion of cells were examined by wound healing and transwell assay. Metastasis of HCC was detected by in vivo experiment. Meanwhile, transcriptome analysis was applied to explore the mechanisms of OXY. The results of transcriptome analysis were validated by in vitro experiment. Furthermore, western blot was used to measure the expression of LC3 and p62 protein. RESULTS OXY significantly inhibited the proliferation, migration and invasion of HCC cells in vitro. OXY suppressed the metastasis of HCC in Balb/c mice with attenuated side effects compared to Doxorubicin. Transcription profiling analysis revealed that OXY may affect autophagy related signaling pathway of HepG2 cells. Western blotting showed that OXY significantly inhibite autophagy by downregulating LC3 and upregulating p62 genes expression. CONCLUSION Our study demonstrated that OXY inhibits the metastasis of HCC by inhibiting autophagy, which suggested OXY to be a candidate for HCC metastasis.

关 键 词：OXYRESVERATROL AUTOPHAGY METASTASIS 

分 类 号：R73[医药卫生—肿瘤]