刺芹侧耳下脚料水溶性细胞壁多糖碱提工艺优化及活性分析  

Alkaline Extraction Condition Optimization for Water Soluble Polysaccharide in Cell Wall of Waste Pleurotus eryngii and Its Immunological Activity in vitro

作　　者：刘艳芳[1] 薛令坤 唐庆九[1] 周帅[1] 颜梦秋[1] 杨焱[1] 吴迪[1] 张忠[1] 张劲松[1] LIU Yanfang;XUE Lingkun;TANG Qingjiu;ZHOU Shuai;YAN Mengqiu;YANG Yan;WU Di;ZHANG Zhong;ZHANG Jingsong(Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Key Laboratory of Applied Mycological Resources and Utilization(South),Ministry of Agriculture,National Engineering Research Center of Edible Fungi,Shanghai Key Laboratory of Agricultural Genetics and Breeding,Shanghai 201403,China)

机构地区：[1]上海市农业科学院食用菌研究所农业部南方食用菌资源利用重点实验室国家食用菌工程技术研究中心上海市农业遗传育种重点实验室

出　　处：《食用菌学报》2019年第4期116-122,共7页Acta Edulis Fungi

基　　金：上海市农委重点攻关项目[沪农科攻字(2015)第6-1-6号]

摘　　要：采用碱提法提取刺芹侧耳(Pleurotus eryngii)下脚料水溶性细胞壁多糖,以得率、多糖含量和β-葡聚糖含量为主要指标,对提取过程中的碱液浓度、料液比、提取温度和提取时间等因素进行考察,优化的提取工艺:1 mol/L的NaOH溶液为提取溶剂,料液比1∶20(g∶mL),提取温度30℃,提取时间4 h,在此条件下,所得水溶性细胞壁多糖组分得率6.25%,多糖含量74.56%,β-葡聚糖含量56.37%。高效凝胶尺寸排阻色谱-多角度激光光散射-示差折光检测仪联用法(HPSEC-MALLS-RI)分析结果显示,该组分主要含4个峰,其中峰1和峰2对应的组分相对分子质量分别为1.36×10^7和2.68×10^6,峰3和峰4对应的组分相对分子质量分别为3.15×10^5和9.82×10^3;体外免疫活性检测结果显示,该水溶性细胞壁多糖组分具有刺激巨噬细胞释放NO和促进小鼠脾淋巴细胞增殖的活性。In this study,NaOH solvents were used to extract cell wall polysaccharides from the water extracted Pleurotus eryngii leftover wastes.Based on yield,water-soluble polysaccharide content andβ-glucan content as evaluation items,the extraction conditions including alkaline concentration,ratio of extraction material to liquid,extraction temperature,and extraction time were optimized.The results showed that the optimized conditions for alkaline extraction of polysaccharide were 1 mol/L NaOH solvent added to leftover wastes at the material to liquid ratio of 1∶20(g∶mL),and then the wastes were extracted twice at 30℃for 4 h.Under the optimized conditions,the yield of the water-soluble cell wall polysaccharide was about 6.25%,and the corresponding contents of the total polysaccharide andβ-glucan were 74.56%and 56.37%,respectively.Based on HPSEC-MALLS-RI,the water-soluble cell wall polysaccharide mainly contained 4 peaks(peak 1-4)with different molecular weight distributions,and their corresponding relative molecular weights from peak 1 to peak 4 were 1.36×10^7,2.68×10^6,3.15×10^5 and 9.82×10^3,respectively.Bioactivity studies in vitro demonstrated that the water-soluble cell wall polysaccharide showed biological activities in increasing NO production by macrophages and stimulating mouse spleen lymphocyte cell proliferation.

关 键 词：刺芹侧耳下脚料 得率 多糖含量 Β-葡聚糖含量 相对分子质量 免疫活性 

分 类 号：R28[医药卫生—中药学]