长裙竹荪多肽抗氧化及免疫调节作用  

Antioxidant and Immunomodulatory Effects of Peptide from Dictyophora indusiata

作　　者：熊川[1] 黄文丽[1] 张利 黎青[1] 李健伟 周贤丹 王玥[3] 朱宇[1] XIONG Chuan;HUANG Wen-li;ZHANG Li;LI Qing;LI Jian-wei;ZHOU Xian-dan;WANG Yue;ZHU Yu(Biotechnology and Nuclear Technology Research Institute,Sichuan Academy of Agricultural Sciences,Chengdu 610061,China;Aba Prefecture Institute of Forestry Science and Technology,Wenchuan 623000,China;Life Sciences College,Sichuan Normal University,Chengdu 610068,China)

机构地区：[1]四川省农业科学院生物技术核技术研究所,四川成都610061 [2]阿坝州林业科学技术研究所,四川汶川623000 [3]四川师范大学生命科学学院,四川成都610068

出　　处：《食品工业科技》2019年第24期51-56,61,共7页Science and Technology of Food Industry

基　　金：四川省高新领域扩展工程(2018GXTZ-001);四川省农业科学院青年基金(2018QNJJ-025)

摘　　要：分离纯化长裙竹荪子实体多肽,对其抗氧化和免疫调节活性进行研究。采用磷酸盐缓冲液提取,分子排阻色谱分离,反相高效液相色谱纯化获得长裙竹荪子实体多肽。通过自由基清除作用,评估其抗氧化活性。通过测定巨噬细胞RAW264.7的吞噬活性、细胞因子分泌能力,评价长裙竹荪多肽的免疫调节作用。结果显示:长裙竹荪多肽得率为0.59%,该多肽具有优良的自由基清除活性,能提高巨噬细胞吞噬能力,显著(P<0.05)促进其细胞因子的分泌,特别是能够迅速促进信息递质NO的分泌和Toll样受体的表达。因此,长裙竹荪多肽可作为一个潜在的免疫调节药物而开发利用。This article mainly studied the extraction,purification,antioxidant and immunomodulatory effects of a novel peptide(DIP)isolated from the fruiting bodies of Dictyophora indusiata. DIP were extracted with phosphate buffer,separated by molecular exclusion chromatography and purified by reversed-phase high performance liquid chromatography(RP-HPLC). The antioxidant activity of DIP was evaluated by free radical scavenging. The immunomodulatory effect of DIP was evaluated by measuring the phagocytic activity and cytokine secretion ability of RAW264.7 cells. The results showed that the extraction yield of DIP was 0.59%. DIP could efficiently scavenge radicals,and had excellent antioxidant activity. Moreover,DIP could promote the phagocytic secretion of macrophages,and promote the secretion of cytokines,especially the secretion of NO and the expression of Toll-like receptors. Therefore,DIP could be exploited as a potential immunomodulatory drug.

关 键 词：长裙竹荪 多肽 抗氧化活性 免疫调节 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]