人参皂苷Rh1对AD小鼠认知障碍的改善作用  

Effect of Ginsenoside Rh1 on Cognitive Impairment in Mice

作　　者：毕云枫[1] 陶伟明 王溪竹[1] 李彤昕 闫璐 孙航 郑明珠[1] 刘景圣[1] BI Yun-feng;TAO Wei-ming;WANG Xi-zhu;LI Tong-xin;YAN Lu;SUN Hang;ZHENG Ming-zhu;LIU Jing-sheng(College of Food Science and Engineering,Jilin Agricultural University,Changchun 130118,China)

机构地区：[1]吉林农业大学食品科学与工程学院

出　　处：《食品工业科技》2019年第24期300-304,共5页Science and Technology of Food Industry

基　　金：吉林省科技厅重点科技研发项目(20180201046NY);长白山学者特聘教授专项资金(00566)

摘　　要：本文研究了人参皂苷Rh1对东莨菪碱导致的小鼠认知障碍的改善作用。小鼠被随机分成六组:空白组:灌胃等剂量生理盐水;阴性对照组:灌胃等剂量生理盐水;阳性对照组:灌胃66.7μg/kg的石杉碱甲;人参皂苷Rh1低、中、高剂量组:灌胃5、10、15 mg/kg。分别进行了跳台试验、避暗试验、Morris水迷宫试验,并测定小鼠脑海马体中乙酰胆碱的含量。结果表明,在跳台试验、避暗试验中,与阴性对照组相比,人参皂苷Rh1低、中、高剂量组小鼠消退试验潜伏期明显延长;消退试验错误次数也明显减少(低、高剂量组P<0.05,中剂量组P<0.01)。在Morris水迷宫试验中,Rh1低、高剂量组找到平台的时间明显缩短(P<0.05);Rh1中剂量组极显著降低(P<0.01),且小鼠的游泳速度明显快于阴性对照组(P<0.05)。实验表明人参皂苷Rh1中剂量组在改善小鼠记忆方面有显著效果,且效果与石杉碱甲相当。The improvement of scopolamine-induced cognitive impairment in mice with ginsenoside Rh1 were studied.The mice were randomly divided into six groups:Normal control group(deionized water),control treated group(deionized water),positive control group(66.7 μg/kg Huperzine A),ginsenoside Rh1 low dose group(5 mg/kg),ginsenoside Rh1 medium dose group(10 mg/kg),ginsenoside Rh1 high dose group(15 mg/kg). The step-down test,passive avoidance test,and Morris water maze test were performed,and the contents of acetylcholine of hippocampus in the mice was determined.Results showed that,in the platform test and the dark test,compared with the negative control group,the latency of the regression test of the ginsenoside Rh1 at low,medium and high dose groups was significantly prolonged,the number of regression test errors was also significantly reduced(low and high dose groups P<0.05,medium dose group P<0.01). In the Morris water maze test,the time to find the platform in the low and high dose groups of Rh1 was significantly shortened(P<0.05),the middle dose group in Rh1 was significantly decreased(P<0.01),and the swimming speed of the mice was significantly faster than the negative control group(P<0.05). The experiments showed that the medium dose of ginsenoside Rh1 had a significant effect on improving memory in mice,and the effect was similar to Huperzine A.

关 键 词：人参皂苷RH1 认知障碍 避暗试验 Morris水迷宫试验 跳台试验 乙酰胆碱含量 

分 类 号：TS201.4[轻工技术与工程—食品科学]