超高效液相色谱法测定不同黄酒中17种氨基酸的分析研究  

Determination of 17 Kinds of Free Amino Acids in Different Huangjiu Products by UPLC

作　　者：田翔[1,2,3] 王君杰 秦慧彬[1,2,3] 乔治军 TIAN Xiang;WANG Junjie;QIN Huibin;QIAO Zhijun(Institute of Crop Variety Resources,Shanxi Academy of Agricultural Sciences,Taiyuan,Shanxi 030031;Key Lab of Crop Gene Resources and Germplasm Enhancement on Loess Plateau,Ministry of Agriculture,Taiyuan,Shanxi 030031;Shanxi Key Lab of Genetic Resources and Genetic Improvement of Minor Crops,Taiyuan,Shanxi 030031,China)

机构地区：[1]山西省农业科学院农作物品种资源研究所,山西太原030031 [2]农业部黄土高原作物基因与种质创制重点实验室,山西太原030031 [3]杂粮种质资源发掘与遗传改良山西省重点实验室,山西太原030031

出　　处：《酿酒科技》2019年第11期74-78,82,共6页Liquor-Making Science & Technology

基　　金：山西省农业科学院特色农业技术攻关项目(YGG17058);国家现代农业产业技术体系项目(CARS-06-13.5-A16)

摘　　要：建立一种快速准确的柱前衍生-超高效液相色谱法,同时测定黄酒中17种游离氨基酸含量。黄酒样以6-氨基喹啉-N-羟基琥珀酰亚胺基氨基甲酸酯(AQC)衍生化处理,以流速为0.7 mL/min经Waters AccQ·TagTM Ultra C18(1.7μm,2.1×100 mm)的色谱柱梯度洗脱,利用二极管阵列检测器,在260 nm波长处检测。结果显示,17种游离氨基酸浓度在25~400μmol/L范围内,峰面积与浓度之间具有良好的线性关系(R2≥0.9993),回收率在94.21%~104.82%。该方法可在11 min内将17种游离氨基酸完全分离,具有准确、快速、重复性好的特点,可用于黄酒中游离氨基酸含量的测定。A rapid method of UPLC coupled with pre-column derivatization has been developed for simultaneous determination of 17 kinds of free amino acids in Huangjiu.Wine samples were derived with 6-aminoquinolyl-N-hydroxysuccinimidylcarbamate(AQC)as derivatization agent,then separated on a Waters AccQ·TagTM Ultra C18 column(1.7μm,2.1×100 mm)at a flow rate of 0.7 mL/min,and finally detected by using PDA detector at the wavelength of 260 nm(UV).The results showed that,the concentration of the 17 amino acids had good correlation(R2≥0.9993)with peak areas in the range of 25μmol/L to 400μmol/L,and the spike recoveries were between 94.21%to 104.82%.All the 17 free amino acids could be separated completely within 11 min by use of this method.The method was featured by accurate and rapid determination with good repeatability.

关 键 词：黄酒 超高效液相色谱法 氨基酸 

分 类 号：TS262.4[轻工技术与工程—发酵工程] TS261.7[轻工技术与工程—食品科学与工程]