微生物菌种选育中基因组重排技术应用的研究进展  

Research Progress of Genome Shuffling Applied in Microbial Strains Screening

作　　者：王珂佳[1,2,3] 邱树毅 WANG Ke-jia;QIU Shu-yi(Department of Light Industry and Chemical Industry,Guizhou Light Industry Technical College,Guiyang 550025,China;Key Laboratory of Fermentation Engineering and Biological Pharmaceutical,Guiyang 550025,China;College of Life Sciences,Guizhou University,Guiyang 550025,China;College of Liquor and Food Engineering,Guizhou University,Guiyang 550025,China)

机构地区：[1]贵州轻工职业技术学院轻工化工系,贵州贵阳550025 [2]贵州省发酵工程与生物制药重点实验室,贵州贵阳550025 [3]贵州大学生命科学学院,贵州贵阳550025 [4]贵州大学酿酒与食品工学院,贵州贵阳550025

出　　处：《食品工业科技》2020年第3期351-356,共6页Science and Technology of Food Industry

基　　金：贵州省工业攻关项目(黔科合GZ字[2014]3012);贵州省重大专项项目(黔科合重大专项字GZ[2013]6009号)

摘　　要：基因组重排(genome shuffling)技术是通过传统诱变育种结合原生质体融合技术开发出的一种新型微生物菌种选育和改良技术,具有易操作、效率高和适用广等特点。本文对基因组重排技术的原理、操作过程及其在增加次生代谢产物产量、增强菌株耐受性和提高底物利用能力等方面的应用进行了综述。Genome shuffling technology is a new type of microbial strain breeding and improvement technology developed through traditional mutation breeding combined with protoplast fusion technology,which has the characteristics of easy operation,high efficiency and wide application. In this paper,the principle and operation process of genome rearrangement technology and its application in increasing the yield of secondary metabolites,enhancing the tolerance of bacterial strains and improving the utilization ability of substrates are reviewed.

关 键 词：微生物 菌种选育 基因重组技术 研究进展 

分 类 号：TS201.1[轻工技术与工程—食品科学]