25株草菇菌株的遗传多样性  

Genetic Diversity of 25 Volvariella volvace Strains

作　　者：马庆芳[1,2] 张丕奇[1,2] 陈鹤[1] 马银鹏[1] 刘佳宁[1,2] 戴肖东[1,2] 韩增华[1,2] 孔祥辉[1,2] 张介驰[1,2] MA Qing-fang;ZHANG Pei-qi;CHEN He;MA Yin-peng;LIU Jia-ning;DAI Xiao-dong;HAN Zeng-hua;KONG Xiang-hui;ZHANG Jie-chi(Institute of Applied Microbiology,Heilongjiang Academy of Sciences,Harbin 150010,China;Institute of Advance Technology,Heilongjiang Academy of Sciences,Harbin 150001,China)

机构地区：[1]黑龙江省科学院微生物研究所,黑龙江哈尔滨150010 [2]黑龙江省科学院高技术研究院,黑龙江哈尔滨150010

出　　处：《中国食用菌》2020年第1期29-34,共6页Edible Fungi of China

基　　金：黑龙江省科学院科学研究基金(KY2018SW03)

摘　　要：为选育出更多适于寒区生产的草菇菌株,从6个草菇生产省引进了25株草菇(Volvariella volvace)菌株,利用表观形态与ISSR分子标记相结合技术对不同来源的草菇菌株进行遗传多样性分析。结果表明,利用c PDA培养基,在30℃恒温培养条件下,依据草菇的表观形态将25个供试草菇菌株划分成4个组群。利用ISSR分子标记聚类分析,相似水平在0.90时可将25个供试草菇菌株细分为8个组群。18号菌株在2种鉴别方法上的归类有差异,有待于栽培农艺性状进一步验证。In order to select more strains of Volvariella volvacea suitable for production in cold regions, 25 V. volvace strains were introduced from 6 provinces. The genetic diversity of 25 V. volvacea strains from different sources was evaluated by apparent morphology compared with ISSR molecular markers. The results indicated that 25 strains were divided into 4 groups according to the apparent morphology of V. volvacea on c PDA medium under the condition of constant temperature culture at30℃. Twenty-five strains of V. volvacea were divided into 8 groups at the similarity level of 0.90 by ISSR molecular marker cluster analysis. The classification of strain 18 is different using two identification methods, which needs further verification of a-gronomic traits.

关 键 词：草菇菌株 表观形态 ISSR分子标记 聚类分析 

分 类 号：S646.9[农业科学—蔬菜学]