以废弃毕赤酵母为高效氮源强化丁酸发酵生产  

Enhanced butyrate production using waste Pichia pastoris as the efficient nitrogen source

作　　者：陈程[1,2] 程文君[1,2] 宫立鹏 丁健 史仲平[1,2] CHEN Cheng;CHENG Wenjun;GONG Lipeng;DING Jian;SHI Zhongping(School of Biotechnology,Jiangnan University,Wuxi 214122,China;The Key Laboratory of Industrial Biotechnology,Ministry of Education(Jiangnan University),Wuxi 214122,China)

机构地区：[1]江南大学生物工程学院,江苏无锡214122 [2]工业生物技术教育部重点实验室(江南大学),江苏无锡214122

出　　处：《食品与发酵工业》2020年第3期22-29,共8页Food and Fermentation Industries

基　　金：国家自然基金(21606106)

摘　　要：为有效利用固态废弃毕赤酵母,高效发酵生产丁酸,建立了以80 g/L玉米淀粉为基础培养基、以废弃毕赤酵母处理液为高效有机氮源,连续补加葡萄糖的丁酸发酵工艺。7 L厌氧发酵罐下,发酵20h,先以1∶4的比例添加250~400 g/L规格的废弃酵母处理液替代昂贵的发酵用复合培养基,再连续补加葡萄糖浓缩液。最终的丁酸质量浓度为45 g/L、得率为40%,丁酸占总酸的比率(butyric acid ratio over total organic acids,B/TA)≥0.90的较高水平。该工艺可以有效地利用来自于废弃酵母处理液中的氨基酸,提高细胞生长速度和浓度,增强丁酸合成所依赖的NADH再生速度,间接改善了丁酸发酵性能。与此同时,实现了废弃生物质的有效利用和资源化。In this study,aiming at effectively utilizing solid waste Pichia pastoris and efficiently producing butyric acid,a novel butyric acid fermentation strategy was developed with 80 g/L corn-starch as the base medium and the pretreated waste yeast suspension as the efficient organic nitrogen source together with consecutive concentrated glucose solution feeding.In a 7L anaerobic fermentor,the waste yeast suspension(250-400g/L specification)was added into fermentation broth with a volume ratio of 1∶4 around 20 h to replace the expensive complex fermentation medium,followed by consecutively feeding concentrated glucose solution.With this strategy,butyric acid concentration,yield and butyric acid ratio over total organic acids(B/TA)reached high levels of 45 g/L,0.40 and 0.90,respectively.This operation strategy could effectively utilize amino acids(4g/L)originated from the waste yeast suspension to increase Clostridium tyrobutyricum growth rate and concentration,enhance butyric acid synthesis dependent NADH regeneration rate under high SO4^2-concentration environment created by waste yeast suspension addition.As a result,butyric acid fermentation performance was indirectly improved,at the same time bio-waste utilization/recirculation was realized.

关 键 词：丁酸发酵 酪丁酸梭菌 有机氮源 还原糖 固态废弃酵母 资源化 

分 类 号：TQ921.4[轻工技术与工程—发酵工程]