保加利亚乳杆菌发酵黄瓜汁产物分析及生物活性评价  

Products analysis of cucumber juice fermentated by Lactobacillus bulgaricus and biological activity evaluation

作　　者：陈琦 赵星 陈小蝶 徐雨晗 廖胜佳 何毅 CHEN Qi;ZHAO Xing;CHEN Xiaodie;XU Yuhan;LIAO Shengjia;HE Yi(Key Laboratory for Deep Processing of Major Grain and Oil,Ministry of Education,Hubei Provincial Key Laboratory for Agricultural Products Processing and Conversion,College of Food Science and Engineering,Wuhan Polytechnic University,Wuhan 430023,China)

机构地区：[1]武汉轻工大学食品科学与工程学院湖北省农产品加工与转化重点实验室大宗粮油精深加工省部共建教育部重点实验室

出　　处：《中国酿造》2020年第1期98-103,共6页China Brewing

基　　金：湖北省自然科学基金(2017CFB309);武汉轻工大学引进人才科研启动项目(2017RZ11)

摘　　要：以黄瓜与鲜牛奶为原料,利用保加利亚乳杆菌(Lactobacillus bulgaricus)对黄瓜牛奶复合物进行发酵。并对发酵液的pH值、总酸、总糖、总酚、乳酸含量和抗氧化性进行分析。同时利用气相色谱-质谱对发酵液的挥发性成分进行分析。采用的发酵工艺为黄瓜汁∶纯牛奶=7∶3(V/V),保加利亚乳杆菌接种量0.01%,初始pH值6.1,发酵温度37℃,发酵时间3 d。在此工艺下对提取的样品进行分析,测得发酵乳的总酸含量为50.5 g/L,总酚含量为0.165 mg/mL,其羟自由基清除率达87%,DPPH自由基清除率达85.12%。总抗氧化能力最高与0.85 mmol/L的Trolox的抗氧化能力相同。发酵液的挥发性成分中,烷类化合物(68.40%)和酯类化合物(10.61%)含量较高,酸类化合物(5.03%)和酮类化合物(0.98%)含量较低。Using cucumber and fresh milk as raw materials,cucumber-milk compound beverage was fermented by Lactobacillus bulgaricus.Then the pH,total acid,total sugar,total phenol,lactic acid content and antioxidant activity of the fermentation broth were analyzed.At the same time,the volatile components of the fermentation broth were analyzed by GC-MS.The fermentation process was cucumber juice to pure milk ratio 7∶3(V/V),L.bulgaricus inoculum 0.01%,initial pH 6.1,fermentation temperature 37℃and fermentation time 3 d.The extracted sample was analyzed under the process,and the total acid content of the fermented milk was 50.5 g/L,the total phenol content was 0.165 mg/ml,the hydroxyl radical scavenging rate was 87%,the DPPH free radical scavenging rate reached 85.12%,and the total antioxidant capacity was the same as the antioxidant capacity of 0.85 mmol/L Trolox.Among the volatile components of fermentation broth,alkanes(68.40%)and esters(10.61%)were higher,acids(5.03%)and ketones(0.98%)were lower.

关 键 词：黄瓜 保加利亚乳杆菌 理化指标 抗氧化性 挥发性成分 

分 类 号：TS252.54[轻工技术与工程—农产品加工及贮藏工程]