Protective effects of catalpol and rhein in murine experimental autoimmune encephalomyelitis via regulation of T helper (Th)1, Th2,Th17, and regulatory T cell responses  

作　　者：Wei Mingyan Yang Tao Li Qian Zhou Dongdong Du Zongpan Fan Yongping 

机构地区：[1]Department of Traditional Chinese Medicine,Beijing Tiantan Hospital,Capital Medical University,Beijing 100050,China [2]Beijing Tongrentang Traditional Chinese Medicine Hospital,Beijing 100051,China [3]Department of Traditional Chinese Medicine,Beijing Tiantan Hospital,Capital Medical University,Beijing 100070,China [4]Beijing Integrative Medicine on Encephalopathy Research Institution,Beijing 100070,China [5]Center for Integrative Medicine,Beijing Ditan Hospital,Capital Medical University,Beijing 100015,China [6]Department of Neurology,Beijing Daxing District Hospital of Integrated Chinese and Western Medicine,Beijing 100076,China

出　　处：《Journal of Traditional Chinese Medicine》2019年第6期809-817,共9页中医杂志（英文版）

基　　金：Supported by the National Natural Science Foundation(No.81973599);Beijing Natural Science Foundation-Key Project of Science and Technology Plan of Beijing Education Commission(KZ/KM/SZ/SM201910025035);The Fund for Beijing Science&Technology Development of Traditional Chinese Medicine(No.QN2018-30)

摘　　要：OBJECTIVE:To examine the effects of catalpol and rhein on pro-and anti-inflammatory responses in C57 BL/6 mice with experimental autoimmune encephalomyelitis(EAE),a model of multiple sclerosis.METHODS:Female C57 BL/6 mice were randomly divided into four groups(n=30):(a)normal salinecontrol,(b)EAE control,(c)EAE+prednisone acetate(PA,6 mg/kg),and(d)EAE+catalpol(40 mg/kg)and rhein(5 mg/kg).EAE was induced by injection of myelin oligodendrocyte glycoprotein 35-55 plus pertussis toxin.Treatments were orally administered daily for 40 d.Disease progression and neurological function were assessed using a semi-quantitative scale of tail and limb paralysis.Brains and spinal cords were collected on Days 6,20,and 40 and assessed for histopathological changes by hematoxylin and eosin staining.Production of interleukin(IL)-2,IL-4,IL-10,and IL-17 A protein was measured by enzyme-linked immunosorbent assay.Expression of the T helper(Th)1-,Th2-,Th17-,and regulatory T cell(Treg)-specific transcription factors T-bet,GATA3,ROR-γt,and Foxp3,respectively,were analyzed by quantitative reverse-transcription polymerase chain reaction and western blot analysis.RESULTS:Combination treatment with catalpol and rhein significantly alleviated the clinical disability and neurological dysfunction of mice with EAE.Catalpol and rhein treatment also reduced the infiltration of pro-inflammatory T cells into pathological lesions;significantly increased the expression of the anti-inflammatory factors GATA3,Foxp3,IL-4,and IL-10;and significantly decreased the expression of the pro-inflammatory factors T-bet,ROR-γt,IL-2,and IL-17 A.CONCLUSION:Catalpol and rhein reduced the neurological disabilities of mice with EAE,at least in part by rebalancing the pro-and anti-inflammatory environment in the brains and spinal cords.OBJECTIVE: To examine the effects of catalpol and rhein on pro-and anti-inflammatory responses in C57 BL/6 mice with experimental autoimmune encephalomyelitis(EAE), a model of multiple sclerosis.METHODS: Female C57 BL/6 mice were randomly divided into four groups(n = 30):(a) normal salinecontrol,(b) EAE control,(c) EAE + prednisone acetate(PA, 6 mg/kg), and(d) EAE + catalpol(40 mg/kg) and rhein(5 mg/kg). EAE was induced by injection of myelin oligodendrocyte glycoprotein 35-55 plus pertussis toxin.Treatments were orally administered daily for 40 d. Disease progression and neurological function were assessed using a semi-quantitative scale of tail and limb paralysis. Brains and spinal cords were collected on Days 6, 20, and 40 and assessed for histopathological changes by hematoxylin and eosin staining. Production of interleukin(IL)-2, IL-4, IL-10, and IL-17 A protein was measured by enzyme-linked immunosorbent assay. Expression of the T helper(Th)1-, Th2-, Th17-, and regulatory T cell(Treg)-specific transcription factors T-bet, GATA3, ROR-γt, and Foxp3, respectively, were analyzed by quantitative reverse-transcription polymerase chain reaction and western blot analysis.RESULTS: Combination treatment with catalpol and rhein significantly alleviated the clinical disability and neurological dysfunction of mice with EAE.Catalpol and rhein treatment also reduced the infiltration of pro-inflammatory T cells into pathological lesions; significantly increased the expression of the anti-inflammatory factors GATA3, Foxp3, IL-4,and IL-10; and significantly decreased the expression of the pro-inflammatory factors T-bet, ROR-γt,IL-2, and IL-17 A.CONCLUSION: Catalpol and rhein reduced the neurological disabilities of mice with EAE, at least in part by rebalancing the pro-and anti-inflammatory environment in the brains and spinal cords.

关 键 词：Multiple sclerosis ENCEPHALOMYELITIS AUTOIMMUNITY CATALPOL RHEIN Th1-Th2 balance Th17 cells 

分 类 号：R285.5[医药卫生—中药学]