基于代谢组学方法的两株酿酒酵母菌胞内差异代谢产物分析  

The analysis of intracellular differential metabolites of two Saccharomyces cerevisiae strains based on metabonomics approach

作　　者：欧科 陈福欣[2] 王婷 冯光文[1] 严贝贝 白巧秀 钱卫东 毛培宏[1] OU Ke;CHEN Fuxin;WANG Ting;FENG Guangwen;YAN Beibei;BAI Qiaoxiu;QIAN Weidong;MAO Peihong(Research Center of Radiation Ecology and Ion Beam Biotechnology,College of Physics Science and Technology,Xinjiang University,Urumqi 830046,China;College of Chemistry and Chemical Engineering,Xi′an University of Science and Technology,Xi′an 710054,China;School of Food and Biological Engineering,Shaanxi University of Science and Technology,Xi′an 710032,China)

机构地区：[1]新疆大学物理科学与技术学院,放射生态与离子束生物技术中心,新疆乌鲁木齐830046 [2]西安科技大学化学与化工学院,陕西西安710054 [3]陕西科技大学食品与生物工程学院,陕西西安710032

出　　处：《食品与发酵工业》2020年第4期39-44,共6页Food and Fermentation Industries

基　　金：国家自然科学基金项目(31760016; 11575149)

摘　　要：为了解不同发酵时期的酿酒酵母菌的胞内小分子化合物的代谢状况,该研究应用代谢组学方法,分析了重组酵母菌株N6076及其原始菌株Kh08的胞内差异代谢产物。UPLC-Q-TOF/MS的检测分析结果表明,2株酵母菌不同发酵时间点的胞内小分子代谢产物存在差异,其种类分别为11和10种,且以高丰度的脂类物质居多,其中甲羟戊酸-5-磷酸和胱氨酸分别为2株酵母菌胞内的显著差异代谢产物。应用LC-MS/MS对甲羟戊酸代谢途径中甲羟戊酸-5-磷酸(MVAP)的检测分析结果表明,重组菌株N6076发酵48 h时胞内MVAP浓度为1.32μg/mL,而原始菌株Kh08在相同发酵时间点未检出MVAP。该研究为进一步认识酵母菌胞内小分子化合物的代谢提供了依据。To further understand the metabolism of intracellular small-molecule compounds in Saccharomyces cerevisiae at different fermentation stages,targeted and non-targeted metabolomic approaches were utilized to analyze the metabolites of recombinant yeast strain N6076 and original strain Kh08 in the present study.The results of UPLC-Q-TOF/MS detection indicated that there were 11 and 10 species of intracellular small-molecule metabolites for strain N6076 and Kh08 at different fermentation time points,respectively,and most of which are lipids with high abundance.Among them,mevalonate-5-phosphate and cystine were the significantly different metabolites of the two strains.The synthesis time of small molecular compounds with molecular weight greater than 900 in metabolites of recombinant strain N6076 was 48 h which later than that of the original strain Kh08.The result detected by LC-MS/MS method showed that the pentenyl mevalonate-5-phosphate(MVAP)concentration was 1.32μg/mL when fermented with strain N6076 for 48 h,while no MVAP was detected at the same fermentation time point of strain Kh08.This study provides a basis for further investigation on the metabolism in yeast.

关 键 词：酵母菌 代谢组 代谢产物 质谱 甲羟戊酸-5-磷酸 

分 类 号：TS261.11[轻工技术与工程—发酵工程]