绿原酸与Ⅲ型细菌素复配对鸡肉中假单胞菌的抑菌机制  

Antibacterial Effect and Mechanism of Chlorogenic Acid Combined with Class Ⅲ Bacteriocin on Pseudomonas fluorescens in Chicken

作　　者：王虹懿 唐敏敏 吴海虹[1] 诸永志[1] 刘芳[1,3] 孙芝兰[1,3] 徐为民[1] 彭景[2] WANG Hongyi;TANG Minmin;WU Haihong;ZHU Yongzhi;LIU Fang;SUN Zhilan;XU Weimin;PENG Jing(Institute of Agricultural Products Processing,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;College of Food Science and Engineering,Yangzhou University,Yangzhou 225127,China;Key Laboratory of Food Quality and Safety of Jiangsu Province,State Key Laboratory Breeding Base,Institute of Food Quality and Nutrition,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China)

机构地区：[1]江苏省农业科学院农产品加工研究所,江苏南京210014 [2]扬州大学食品科学与工程学院,江苏扬州225127 [3]江苏省农业科学院农产品质量与营养安全研究所,省部共建国家重点实验室培育基地,江苏省食品质量安全重点实验室,江苏南京210014

出　　处：《肉类研究》2020年第2期1-6,共6页Meat Research

基　　金：江苏省农业自主创新基金项目（CX（19）3073）;江苏省克氏原鳌虾产业技术体系建设专项（JFRS-03）;国家自然科学基金面上项目（31972140）;江苏省政策引导类计划苏北科技专项（SZ-SQ2018033）

摘　　要：荧光假单胞菌是引起冷鲜鸡肉腐败变质的优势腐败菌,可降解肉品中的蛋白质、脂肪等物质,产生多种腐败代谢产物,进而使肉类食品风味和品质发生劣变。采用体外抑菌实验研究绿原酸(chlorogenic acid,CA)、Ⅲ型细菌素Helveticin-M及二者复配对荧光假单胞菌的抑菌效果;通过扫描电子显微镜观察不同处理对荧光假单胞菌外部形态结构的影响,通过激光共聚焦显微镜观察、测定胞外ATP含量、胞外蛋白和核酸外渗,研究CA或Helveticin-M对指示菌细胞膜渗透性的影响。结果表明:与CA或Helveticin-M单独作用相比,二者复配后抑菌作用显著增强(P<0.05);绿原酸与Helveticin-M复配处理可显著破坏荧光假单胞菌形态,增强细胞膜渗透性,加剧胞内物质外泄,最终加速荧光假单胞菌死亡。Pseudomonas fluorescein is the dominant spoilage bacterium in chilled fresh chicken,which can degrade protein,fat and other substances in meat,producing a variety of spoilage metabolites,which deteriorate the flavor and quality of meat.The objective of this study was to explore the antimicrobial effect of Helveticin-M,a classⅢbacteriocin and/or chlorogenic acid(CA)against Pseudomonas fluorescens by in vitro experiments.The morphological structure of Pseudomonas fluorescens was observed by a scanning electron microscope(SEM).To evaluate the effect of Helveticin-M and CA on the cell membrane permeability of the indicator strain,confocal laser scanning micrographs(CLSM)were recorded and extracellular ATP and protein contents as well as leakage of ultraviolet absorbing substances were measured.The results showed that the combined antibacterial effect of Helveticin-M and CA was markedly increased compared with either agent alone(P<0.05).Furthermore,the combined treatment significantly damaged the morphology of Pseudomonas fluorescens,increased the membrane permeability,promoted the leakage of intracellular substances,and finally accelerated cell death.

关 键 词：绿原酸 Ⅲ型细菌素Helveticin-M 荧光假单胞菌 抑菌机制 

分 类 号：TS201.3[轻工技术与工程—食品科学]