莲蓬壳中挥发油成分的定性和定量分析  

Qualitative and Quantitative Analysis of Volatile Oil Components from Lotus (Nelumbo nucifera Gaertn.) Receptacle

作　　者：张建梅[1,2] 潘婷 何静 顾倩 尚志 胡卫成 ZHANG Jian-mei;PAN Ting;HE Jing;GU Qian;SHANG Zhi;HU Wei-cheng(Jiangsu Collaborative Innovation Center of Regional Modern Agriculture&Environmental Protection,Huaiyin Normal University,Huaian 223300,China;College of Food Science and Pharmacology,Xinjiang Agricultural University,Urunqi 830052,China)

机构地区：[1]淮阴师范学院,江苏省高校区域现代农业与环境保护协同创新中心,江苏淮安223300 [2]新疆农业大学食品科学与药学学院,新疆乌鲁木齐830052

出　　处：《保鲜与加工》2020年第2期214-218,共5页Storage and Process

基　　金：江苏高校品牌专业建设工程资助项目(PPZY2015A018)。

摘　　要：为研究莲蓬壳中挥发油的成分及含量,以莲蓬壳为试材,采用索氏提取法从莲蓬壳中提取出挥发油,并通过气相色谱-质谱联用技术(GC-MS)结合计算机检索系统对其挥发油的主要化学成分进行定性和定量分析。结果表明,莲蓬壳挥发油共鉴定出32种化学成分,占总挥发性物质含量的99.83%,主要分为烃类、醇类、酮类、酯类、酚类、酸类等7类化合物。通过面积归一化法对其含量进行分析,研究发现莲蓬壳挥发油的主要成分为α-香树精、β-香树精、12-齐墩果烯-3-酮、β谷甾醇油酸酯、谷甾醇、乙酸环阿屯酯、羽扇烯酮等,其中,α-香树精含量最高,为27.93%。本研究确定了莲蓬壳中挥发油的主要化合物组成,为合理开发和利用莲蓬壳提供理论依据。In order to study the composition and content of volatile oil in lotus receptacle,Soxhlet extraction method was used to extract volatile oil from lotus receptacle.The main chemical constituents of the volatile oils were qualitatively and quantitatively analyzed by gas chromatography-mass spectrometry(GC-MS)combined with computer retrieval system.The results showed that a total of thirty-two components were identified from the volatile oil of lotus receptacle and accounted for 99.83%,which mainly divided into seven classes,including hydrocarbons,alcohols,ketones,esters,phenols,acids,and so on.The content of the components was calculated by peak area normalization method.The main components of the volatile oil of lotus receptacle were alpha-amyrin,beta-amyrin,12-oleanen-3-one,beta-sitosteryl oleate,sitosterol,cycloartenyl acetate,Lupenone,etc,among which alpha-amyrin was the most abundant with a content of 27.93%.The study confirmed the composition of the main compounds of volatile oil in lotus receptacle,which provided a theoretical basis for the rational development and utilization of lotus receptacle.

关 键 词：莲蓬壳 挥发油 气相色谱-质谱联用技术 定性和定量分析 

分 类 号：TS201.2[轻工技术与工程—食品科学]