类蛋白反应修饰的牡蛎肽锌结合物的生物利用性  

Bioavailability of Oyster-derived Zinc Binding Peptide Modified by Plastein Reaction

作　　者：王再扬 曹玉惠 赵元晖[1] 崔永日 薛长湖[1] 曾名湧[1] Wang Zaiyang;Cao Yuhui;Zhao Yuanhui;Cui Yongri;Xue Changhu;Zeng Mingyong(College of Food Science and Engineering,Ocean University of China,Qingdao 266003,Shandong;Kangmai Chen Biotechnology Co.,Ltd.,Qingdao 266105,Shandong)

机构地区：[1]中国海洋大学食品科学与工程学院,山东青岛266003 [2]青岛康迈臣生物科技有限责任公司,山东青岛266105

出　　处：《中国食品学报》2020年第3期46-51,共6页Journal of Chinese Institute Of Food Science and Technology

基　　金：山东省自然科学基金项目(ZR2019MC014);国家贝类产业技术体系项目(CARS-49)。

摘　　要：通过类蛋白反应(Plastein)修饰制备具有较高锌离子结合能力的牡蛎源肽(EVPPEEH),其锌结合能力为161 mg/g。以缺锌型SD大鼠为试验模型,以牡蛎酶解肽-锌结合物OPZ、类蛋白反应修饰的牡蛎酶解肽-锌结合物LPZ和ZnSO4(ZS)为对照,研究牡蛎源肽(EVPPEEH)与锌的结合物(MZ)在体内的生物利用率和生物利用途径。结果表明:MZ组大鼠血清锌水平,股骨锌储备量及肝脏锌含量和肾脏锌含量均显著高于各对照组。脾脏锌含量MZ组和LPZ组恢复程度相似,均显著高于OPZ组和ZS组。MZ组和各对照组大鼠体重和摄食量均显著增加,效果相似。大鼠小肠转锌蛋白(ZnT1)的表达量MZ组与无机锌组差异不是很明显,而小肽转运蛋白(PepT1)的表达量MZ组最高,相对于ZS组上调了20%,显著高于各对照组,说明大鼠小肠对MZ的吸收利用一部分是通过传统锌离子途径,另一部分是通过小肽途径。MZ有望成为一种新型高效的补锌制剂。The oyster-derived peptide(EVPPEEH)with higher zinc ion binding capacity was prepared by a proteinoid reaction(Plastein)modification with a zinc binding capacity of 161 mg/g.Zinc-deficient SD rats were used as experimental models,and oyster zinc-binding peptide OPZ and zinc-binding peptide modified by plastein reaction LPZ and ZnSO4(ZS)were used as control to study the vivo bioavailability and bioavailability of the oyster-derived metal chelating(EVPPEEH)and zinc conjugate(MZ).The results showed that the levels of serum zinc,the reserves of femoral zinc and the contents of zinc in the liver and the kidneys in MZ group were significantly higher than those in the control group.Spleen zinc levels MZ group and LPZ group similar recovery,were significantly higher than OPZ group and ZS group.The body weight and food intake of MZ group and each control group were significantly increased,the effect is similar.The expression of ZnT1 in small intestine was not significantly different between MZ group and inorganic Zn group,while the expression of PepT1 was the highest in MZ group,which was 20%higher than that in ZS group,which was significantly higher than that in ZS group each control group.This shows that the absorption and utilization of MZ by the small intestine of rats are partly through the traditional zinc ion pathway and partly through the small peptide pathway.MZ is expected to become a new and efficient zinc supplement.

关 键 词：牡蛎 肽锌结合物 类蛋白反应 生物利用性 转锌蛋白 

分 类 号：TS201.2[轻工技术与工程—食品科学]