4株抗致病菌Canidia albicans YTS-03的潜在工程酵母菌抗性及其抗菌能力  

The Stress Resistance and Antibacterial Ability of 4 Kinds of Potential Engineering Yeasts to Pathogenic Canidia albicans YTS-03

作　　者：谭春明[1,2] 王麟 赵旺 吴开畅[1,2] 温为庚 陈旭[1] 于刚 TAN Chun-ming;WANG Lin;ZHAO Wang;WU Kai-chang;WEN Wei-geng;CHEN Xu;YU Gang(Tropical Aquaculture Research and Development Center of South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Sanya 572018,China;Key Laboratory of South China Sea Fishery Resources Exploitation Utilization,Ministry of Agriculture and Rural Affairs,Guangzhou 510300,China;Beihang-goertec Microelectronics Institute,Beihang Qingdao Research Institute,Qingdao 266041,China)

机构地区：[1]中国水产科学研究院南海水产研究所,热带水产研究开发中心,海南三亚572018 [2]农业农村部南海渔业资源开发利用重点实验室,广东广州510300 [3]北京航空航天大学青岛研究院,北航歌尔微电子研究院,山东青岛266041

出　　处：《食品工业科技》2020年第7期89-94,101,共7页Science and Technology of Food Industry

基　　金：现代农业产业技术体系建设专项资金(CARS-49);海南省自然科学基金青年基金项目(319QN341);海南省自然科学基金青年基金项目(318QN304);三亚市院地科技合作项目(2018YD19)。

摘　　要：为了获得对致病菌Canidia albicans YTS-03具有强抗性的嗜杀酵母工程菌,以开发抗真菌制剂。对所筛选的4株优良菌株PA1、SY3-1A、DD21-2、WM1进行了耐高温性、耐酸碱性、耐盐性、抗生素抗性及抗菌能力检测。结果表明:PA1、SY3-1A、DD21-2、WM1对温度和环境pH的适应范围较宽,可以在12~37℃及pH3~9的条件下生长繁殖,并且其耐盐性存在较大的差异,分别为10%、8%、7%和4%;温度、pH和盐度均对实验菌株生长有显著影响(P<0.05)。4株菌对抗菌素较为敏感,对伊曲康唑和低浓度(5μg/mL)的制霉菌素或低浓度(5μg/mL)的氟康唑有抗性,并且4株嗜杀酵母菌株具有不同的抗药性特征。另外,4株嗜杀酵母菌株PA1、SY3-1A、DD21-2、WM1均能杀死白色假丝酵母、二尖梅奇酵母和热带假丝酵母,而且表现出高嗜杀活力,其菌液嗜杀因子对C.albicans YTS-03菌株的抑菌圈直径分别达到23.0、14.1、22.3和21.5 mm。In order to obtain an engineered killer yeast which was highly resistant to the fungal pathogen Canidia albicans YTS-03,and to develop antifungal preparations,four kinds of excellent strains for resistance of high temperature,acid and alkali,salt,antibiotic,and antibacterial ability were tested.The results showed that the strain PA1,SY3-1A,DD21-2 and WM1 had wide adaptability to temperature and environmental pH,which could grow under the conditions of 12~37℃and pH3~9.The salt tolerances of the strains were quite different,9%(PA1),8%(SY3-1A),7%(DD21-2),and 4%(WM1)respectively.The temperature,environmental pH,and salt concentration all had significant effects on the growth of strains(P<0.05).The killer yeasts were more sensitive to antibiotics,having resistance to itraconazole and mycin with low concentration(5μg/mL)or chloroconazole,and different killer yeast strains had different drug resistance characteristics.These four test strains could kill Candida albicans,Metschnikowiabicuspidata and Candida tropicalis,and with strong killing activity,the diameter of inhibition zone of strain PA1、SY3-1A、DD21-2、WM1 against C.albicans YTS-03 reached 23.0,14.1,22.3 and 21.5 mm,respectively.

关 键 词：嗜杀酵母 致病菌 抗逆性 抗菌能力 

分 类 号：TS201.3[轻工技术与工程—食品科学]