Antitumor activity of nervosine Ⅶ,and the crosstalk between apoptosis and autophagy in HCT116 human colorectal cancer cells  

作　　者：HUANG Shuai ZHAO Shuang-Mei SHAN Lian-Hai ZHOU Xian-Li 

机构地区：[1]School of Life Science and Engineering,Southwest Jiaotong University,Chengdu 610031,China

出　　处：《Chinese Journal of Natural Medicines》2020年第2期81-89,共9页中国天然药物（英文版）

基　　金：This work was supported by the National Natural Science Foundation of China(No.81402803).

摘　　要：Nervosine Ⅶ is one of the known saturated pyrrolizidine alkaloids isolated from the plant of Liparis nervosa.This is first study to investigate the antitumor activity of nervosine Ⅶ in vitro,and the results indicated that nervosine Ⅶ induced autophagy and apoptosis in HCT116 human colorectal cancer cells.Mechanistic studies showed that nervosine Ⅶ-induced apoptosis was associated with the intrinsic pathway by the activation of caspase-9,-3 and-7.Autophagy induced by nervosine Ⅶ was characteristic with the regulation of autophagic markers including the increase of LC3-Ⅱ and beclin 1 proteins,and the decrease of p62 protein.Nervosine Ⅶ simultaneously induced autophagy and apoptosis by activated MAPKs signaling pathway including JNK,ERK1/2 and p38,suppressing the p53 signaling pathway.Nervosine VII is one of the known saturated pyrrolizidine alkaloids isolated from the plant of Liparis nervosa. This is first study to investigate the antitumor activity of nervosine VII in vitro, and the results indicated that nervosine VII induced autophagy and apoptosis in HCT116 human colorectal cancer cells. Mechanistic studies showed that nervosine VII-induced apoptosis was associated with the intrinsic pathway by the activation of caspase-9,-3 and-7. Autophagy induced by nervosine VII was characteristic with the regulation of autophagic markers including the increase of LC3-II and beclin 1 proteins, and the decrease of p62 protein.Nervosine VII simultaneously induced autophagy and apoptosis by activated MAPKs signaling pathway including JNK, ERK1/2 and p38, suppressing the p53 signaling pathway.

关 键 词：Liparis nervosa PYRROLIZIDINE ALKALOIDS NervosineⅦ AUTOPHAGY APOPTOSIS HCT116 cells 

分 类 号：R965[医药卫生—药理学]