过表达热激蛋白提高酿酒酵母的耐高温高乙醇性能  

Overexpression of heat shock protein enhances heat and ethanol tolerance of Saccharomyces cerevisiae

作　　者：陈胜杰 高翔 袁戎宇 CHEN Shengjie;GAO Xiang;YUAN Rongyu(Guangdong Cleamol Technology Co.,Ltd.,Foshan 528000,China)

机构地区：[1]广东怡和科洁科技有限公司,广东佛山528000

出　　处：《发酵科技通讯》2020年第1期11-17,共7页Bulletin of Fermentation Science and Technology

摘　　要：传统的酿酒酵母对高温高乙醇质量分数敏感,增加工业化生产成本。采用分子生物学手段,过表达关键基因sym1和hsp104,强化热激蛋白的表达,使出发菌的耐高温、耐高乙醇能力显著提升,最终使改造菌在乙醇质量分数20%,发酵温度40℃的条件下,能够保持接近35%的细胞存活率,在同条件下,出发菌株的存活率仅为2.1%。研究结果表明:过表达关键基因sym1和hsp104可以增加酿酒酵母的耐高温和耐高乙醇能力。代谢流分析表明笔者的改造方案能增强合成乙醇的碳流量。The traditional Saccharomyces cerevisiae is sensitive to high temperature and high ethanol concentration,increasing industrial production costs.This research aimed to improve the S.cerevisiae resistance to high temperature and high ethanol concentration and provide experience for industrialization.By means of molecular biology,we overexpressed the key genes sym1 and hsp104,and enhanced expression of heat shock protein.Finally,the modified bacteria could maintain the cell survival rate of 35%under the conditions of 20%ethanol concentration and fermentation temperature 40℃.The results suggested overexpressed key genes sym1 and HSP 104 could increase the resistance to high temperature and high ethanol of S.cerevisiae,and the metabolic flow analysis showed that the modification could enhance the carbon flow of synthetic ethanol.

关 键 词：酿酒酵母 质粒过表达 耐高温 耐乙醇 代谢流分析 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]