新疆维吾尔族肠道中高产胞外多糖双歧杆菌的筛选及其抗氧化活性  

Screening for and Antioxidant Activities of High Exopolysaccharide-Producing Bifidobacterium from the Intestinal Tract of Uygur Infants and Their Mothers in Xinjiang

作　　者：蔡静静 徐晓裕 张艳[1] 张亚川 魏小晶 阚泽宇 倪永清[1] CAI Jingjing;XU Xiaoyu;ZHANG Yan;ZHANG Yachuan;WEI Xiaojing;KAN Zeyu;NI Yongqing(Food College,Shihezi University,Shihezi 832000,China)

机构地区：[1]石河子大学食品学院,新疆石河子832000

出　　处：《食品科学》2020年第8期144-151,共8页Food Science

基　　金：石河子大学高层次人才启动项目(CZX201432R)。

摘　　要：使用Wilkins-Chalgren厌氧菌琼脂和BSM培养基作为筛选平板,结合重复基因外回文序列-聚合酶链式反应和16S rRNA序列分析,对新疆维吾尔族婴儿及其母亲粪便中的双歧杆菌(Bifidobacterium)进行分离鉴定,并筛选出高产胞外多糖的双歧杆菌,测定其多糖的抗氧化活性以及菌株的耐受性和黏附性。结果显示,20份粪便样品中共分离出52株双歧杆菌,其中假小链双歧杆菌(B.pseudocatenulatum)14株,假长双歧杆菌(B.pseudolongum)8株,两歧双歧杆菌(B.bifidum)9株,短双歧杆菌(B.breve)7株,长双歧杆菌婴儿亚种(B.longum subsp.infantis)5株,动物双歧杆菌乳亚种(B.animal subsp.lactis)6株以及长双歧杆菌(B.longum)3株。经过表型初筛和苯酚-硫酸法复筛,共筛选出7株高产胞外多糖的双歧杆菌,37℃发酵36 h后胞外多糖产量均可达400 mg/L以上。抗氧化活性实验结果表明7株双歧杆菌所产的胞外多糖对过氧化氢自由基、超氧阴离子自由基、羟自由基和1,1-二苯基-2-三硝基苯肼自由基均有一定的清除能力。此外,菌株BF66-16相较于其他几株双歧杆菌具有较强的胃肠液耐受性以及黏附性,因此来源于婴儿粪便的BF66-16可以作为潜在的抗氧化菌株应用于制药和食品工业中。This study aimed to isolate and identify Bifidobacterium strains from the feces of Uygur infants and their mothers’in Xinjiang.Using Wilkins-Chalgren anaerobic agar and BSM medium plates,Bifidobacterium strains were isolated and they were identified by repetitive extragenic palindromic-polymerase chain reaction(rep-PCR)fingerprinting and 16S rRNA gene analysis.High-yield expolysaccharides(EPS)producing strains were selected from these isolates and the antioxidant activities of the EPS produced by the selected strains as well as their tolerance and auto-adhesion ability were evaluated.The results showed that a total of 52 stains of Bifidobacterium were isolated from 20 feces samples and they belonged to B.pseudocatenulatum(14 strains),B.pseudolongum(8 strains),B.bifidum(9 strains),B.breve(7 strains),B.longum subsp.infantis(5 strains),B.animal subsp.lactis(6 strains)and B.longum(3 strains).respectively.Through phenotype screening and secondary screening using the phenol sulfuric acid method,we found 7 strains of Bifidobacterium with higher EPS yield.The yield of EPS produced by each of the 7 strains was more than 400 mg/L after 36 h fermentation at 37℃.The EPS had the ability to scavenge H2O2,superoxide anion,hydroxyl and 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical.Besides,strain BF66-16 had stronger tolerance to gastrointestinal juice and auto-adhesion ability than the other six strains.Thus,BF66-16 can be used as a potential antioxidant strain in the pharmaceutical and food industries.

关 键 词：双歧杆菌 重复基因外回文序列-聚合酶链式反应 胞外多糖 抗氧化活性 

分 类 号：Q938[生物学—微生物学]